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Histone H3.3 antibody

Anti-Histone H3.3 antibody used in Western Blot (WB). GTX101754
Anti-Histone H3.3 antibody used in Western Blot (WB). GTX101754
Anti-Histone H3.3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101754

Cat No. GTX101754

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

IMR32 , PC-12 , Rat2

Predict Reactivity

Mouse, Zebrafish, Rabbit, Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Histone H3.3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037176

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H3.3A antibody , H3F3 antibody , H3F3B antibody , MGC87782 antibody , MGC87783 antibody , H3F3A antibody , histone H3.3 antibody , "H3 histone , family 3A antibody"

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-Histone H3.3 antibody used in Western Blot (WB). GTX101754

GTX101754 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32 15% SDS PAGE
GTX101754 diluted at 1:1000

Anti-Histone H3.3 antibody used in Western Blot (WB). GTX101754

GTX101754 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3 antibody (GTX101754) diluted at 1:500.

Anti-Histone H3.3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101754

GTX101754 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Histone H3.3(GTX101754) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169