*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
IMR32 , PC-12 , Rat2
Mouse, Zebrafish, Rabbit, Bovine, Pig, Xenopus laevis(>80% identity)
0.1M Tris-Glycine (pH7), 10% Glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.08 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human Histone H3.3. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
H3.3A antibody , H3F3 antibody , H3F3B antibody , MGC87782 antibody , MGC87783 antibody , H3F3A antibody , histone H3.3 antibody , "H3 histone , family 3A antibody"
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq]
GTX101754 WB Image
Sample (30 ug of whole cell lysate)
A: IMR32 15% SDS PAGE
GTX101754 diluted at 1:1000
GTX101754 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3 antibody (GTX101754) diluted at 1:500.
GTX101754 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Histone H3.3(GTX101754) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.