GeneTex
United States (US)

Histone H3.3B antibody

GTX115549_42655_20161205_IHC-P_R_18121410_510.jpg
GTX115549_42656_20161228_IFA_18121410_830.jpg
GTX115549_41080_20150313_IHC_M_18121410_542.jpg
GTX115549_41080_IHC_M_18121410_846.jpg
GTX115549_40212_IHC_18121410_841.jpg
GTX115549_42656_20161205_IHC-P_M_18121410_779.jpg
GTX115549_41080_20150313_IHC_M_2_18121410_150.jpg
GTX115549_42655_20161117_WB_18121410_876.jpg
GTX115549_40212_WB_R_brain_18121410_444.jpg
GTX115549_42656_20161205_IHC-P_R_18121410_765.jpg
GTX115549_41080_IHC_M_2_18121410_630.jpg
GTX115549_42655_20161205_IHC-P_M_18121410_903.jpg
GTX115549_40212_WB_M_brain_18121410_919.jpg

Cat No. GTX115549

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Histone H3.3B Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H3.3B antibody, H3F3A antibody, H3F3B antibody, histone H3.3 antibody, H3 histone, family 3A antibody, H3 histone, family 3B (H3.3B) antibody

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the histone H3 family. [provided by RefSeq]

Research Area

DATA IMAGES
GTX115549_42655_20161205_IHC-P_R_18121410_510.jpg

GTX115549 IHC-P Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Histone H3.3B antibody (GTX115549) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115549_42656_20161228_IFA_18121410_830.jpg

GTX115549 ICC/IF Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3.3B protein stained by Histone H3.3B antibody (GTX115549) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.

GTX115549_41080_20150313_IHC_M_18121410_542.jpg

GTX115549 IHC-P Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Histone H3.3B antibody (GTX115549) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115549_41080_IHC_M_18121410_846.jpg

GTX115549 IHC-P Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Histone H3.3B antibody (GTX115549) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115549_40212_IHC_18121410_841.jpg

GTX115549 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Histone H3.3B(GTX115549) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115549_42656_20161205_IHC-P_M_18121410_779.jpg

GTX115549 IHC-P Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H3.3B antibody (GTX115549) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115549_41080_20150313_IHC_M_2_18121410_150.jpg

GTX115549 IHC-P Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse spinal cord by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spinal cord.
Histone H3.3B antibody (GTX115549) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115549_42655_20161117_WB_18121410_876.jpg

GTX115549 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3B antibody (GTX115549) diluted at 1:10000.

GTX115549_40212_WB_R_brain_18121410_444.jpg

GTX115549 WB Image

Histone H3.3B antibody detects H3F3B protein by western blot analysis.
A. 50 µg rat brain lysate/extract
15% SDS-PAGE
Histone H3.3B antibody (GTX115549) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115549_42656_20161205_IHC-P_R_18121410_765.jpg

GTX115549 IHC-P Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Histone H3.3B antibody (GTX115549) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115549_41080_IHC_M_2_18121410_630.jpg

GTX115549 IHC-P Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Histone H3.3B antibody (GTX115549) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115549_42655_20161205_IHC-P_M_18121410_903.jpg

GTX115549 IHC-P Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Histone H3.3B antibody (GTX115549) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115549_40212_WB_M_brain_18121410_919.jpg

GTX115549 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX115549 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169