Histone H3.3B antibody

Name: Histone H3.3B antibody
Brand: GeneTex
SKU: GTX115549
Price: 399.00 USD

Anti-Histone H3.3B antibody used in IHC (Paraffin sections) (IHC-P). GTX115549

Anti-Histone H3.3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115549

Anti-Histone H3.3B antibody used in Western Blot (WB). GTX115549

Cat. No. GTX115549

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all Histone H3 products

View product citations for antibody GTX115549 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Mouse brain , rat brain , 293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Histone H3.3B Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624805

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

H3 histone family member 3B , H3.3B

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17. [provided by RefSeq, Oct 2015]

Database

Gene ID: 3021 H3F3B
UniProt: P84243 H3F3B

Research Area

DATA IMAGES

GTX115549 IHC-P Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Histone H3.3B antibody (GTX115549) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115549 IHC-P Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Histone H3.3B antibody (GTX115549) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115549 IHC-P Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H3.3B antibody (GTX115549) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115549 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Histone H3.3B(GTX115549) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115549 ICC/IF Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3.3B protein stained by Histone H3.3B antibody (GTX115549) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.

GTX115549 WB Image

Histone H3.3B antibody detects H3F3B protein by western blot analysis.
A. 50 μg rat brain lysate/extract
15% SDS-PAGE
Histone H3.3B antibody (GTX115549) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115549 IHC-P Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Histone H3.3B antibody (GTX115549) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115549 IHC-P Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse spinal cord by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spinal cord.
Histone H3.3B antibody (GTX115549) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115549 IHC-P Image

Histone H3.3B antibody detects Histone H3.3B protein at nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Histone H3.3B antibody (GTX115549) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115549 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX115549 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115549 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3B antibody (GTX115549) diluted at 1:10000.