Histone H3 (K27M Mutant) antibody [HL2794]

Cat. No. GTX639662

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2794
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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Datasheet

View product citations for antibody GTX639662 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of Histone H3 (K27M Mutant).

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX639662 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (K27M Mutant) antibody [HL2794] (GTX639662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX639662 ICC/IF Image

Histone H3 (K27M Mutant) antibody [HL2794] detects Histone H3 (K27M Mutant) protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: Histone H3 (K27M Mutant) stained by Histone H3 (K27M Mutant) antibody [HL2794] (GTX639662) diluted at 1:500.
Red: DDDDK tag, a marker, stained by DDDDK tag antibody [GT1216] (GTX636630) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty

Available soon.

Custom Conjugations

Histone H3 (K27M Mutant) antibody [HL2794]

Cat. No. GTX639662

Host

Clonality

Clone Name

Isotype

Application

Reactivity