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Histone H3 antibody - ChIP grade

Anti-Histone H3 antibody - ChIP grade used in Western Blot (WB). GTX60349
Anti-Histone H3 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60349
Anti-Histone H3 antibody - ChIP grade used in ELISA (ELISA). GTX60349

Cat. No. GTX60349

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, ChIP assay

Reactivity

 Human, Zebrafish
Package
50 μl ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ELISA 1:10,000
ChIP assay 1-5 μg
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Two KLH-conjugated synthetic peptides containing an unmodified sequence from the central part and from the C-terminus of histone H3, respectively.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3 antibody - ChIP grade used in Western Blot (WB). GTX60349

GTX60349 WB Image

WB analysis of whole cell extracts from HeLa cells (25 μg) using GTX60349 Histone H3 antibody - ChIP grade.
Dilution : 1:500

Anti-Histone H3 antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60349

GTX60349 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60349 Histone H3 antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5, and 10 μl per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as negative controls, and for the inactive MYOD1 and the Sat2 satellite repeat, used as positive controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Anti-Histone H3 antibody - ChIP grade used in ELISA (ELISA). GTX60349

GTX60349 ELISA Image

ELISA analysis of peptides used for immunization using GTX60349 Histone H3 antibody - ChIP grade.

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Package List Price ($)
$ 399