Histone H3 antibody [RM186]

Cat. No. GTX60910

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM186
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human
Package	100 μg ($449)

See all Histone H3 products

Datasheet

View product citations for antibody GTX60910 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1 μg/mL - 2 μg/mL
ICC/IF	0.5 μg/mL - 2 μg/mL
ELISA	0.5 μg/mL - 1 μg/mL

Not tested in other applications.

Product Note

This antibody recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

An unmodified peptide corresponding to the N-terminus of Histone H3.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60910 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60910 Histone H3 antibody [RM186].
Red : Primary antibody
Green : Actin

GTX60910 WB Image

WB analysis of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated and recombinant histone H3.1 protein using GTX60910 Histone H3 antibody [RM186].
Dilution : 1μg/ml

GTX60910 Image

The GTX60910 recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, mono- methylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 449

Qty

Histone H3 antibody [RM186]

Cat. No. GTX60910

Host

Clonality

Clone Name

Isotype

Application

Reactivity