The GTX60910 recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, mono- methylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3.

WB analysis of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated and recombinant histone H3.1 protein using GTX60910 Histone H3 antibody [RM186].
Dilution : 1μg/ml

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60910 Histone H3 antibody [RM186].
Red : Primary antibody
Green : Actin