Histone H3 antibody

Name: Histone H3 antibody
Brand: GeneTex
SKU: GTX122150
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Histone H3 antibody used in Western Blot (WB). GTX122150

Anti-Histone H3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122150

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122150

Cat. No. GTX122150

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Drosophila
Package	100 μl ($399), 25 μl ($169)

See all Histone H3 products

Datasheet
Document

View product citations for antibody GTX122150 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720300

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX122150 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122150) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX122150 ICC/IF Image

HIST1H3A antibody detects HIST1H3A protein at chromosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: HIST1H3A protein stained by HIST1H3A antibody (GTX122150) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX122150 IHC-P Image

Histone H3.1 antibody detects Histone H3.1 protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3.1 antibody (GTX122150) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX122150 IHC-P Image

GTX122150 ICC/IF Image

Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3 protein stained by Histone H3 antibody (GTX122150) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

GTX122150 IHC-P Image

Histone H3.1 antibody detects Histone H3.1 protein at nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Histone H3.1 antibody (GTX122150) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX122150 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122150) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX122150 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122150) diluted at 1:5000.

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REVIEW