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Histone H3 antibody

Anti-Histone H3 antibody used in Western Blot (WB). GTX127985
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX127985

Cat No. GTX127985

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

A375

Predict Reactivity

Mouse, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H3/A antibody , H3FA antibody , HIST1H3B antibody , HIST1H3C antibody , HIST1H3D antibody , HIST1H3E antibody , HIST1H3F antibody , HIST1H3G antibody , HIST1H3H antibody , HIST1H3I antibody , HIST1H3J antibody , HIST1H3A antibody , histone H3/j antibody , histone H3/i antibody , histone H3/d antibody , histone H3/a antibody , histone H3/h antibody , histone H3/k antibody , histone H3/l antibody , histone H3.1 antibody , H3 histone family , member A antibody , histone H3/c antibody , histone 1 , H3a antibody , histone H3/f antibody , histone H3/b antibody , histone cluster 1 , H3a antibody

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-Histone H3 antibody used in Western Blot (WB). GTX127985

GTX127985 WB Image

Histone H3 antibody detects Histone H3 protein by Western blot analysis.
A. 30 μg A375 whole cell lysate/extract
15 % SDS-PAGE
Histone H3 antibody (GTX127985) dilution: 1:5000

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX127985

GTX127985 IHC-P Image

Histone H3 antibody detects Histone H3 protein at cytosol and nucleus on AGS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded AGS xenograft.
Histone H3 antibody (GTX127985) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169