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Histone H3 antibody

Anti-Histone H3 antibody used in Western Blot (WB). GTX127985
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX127985
Anti-Histone H3 antibody used in Western Blot (WB). GTX127985

Cat. No. GTX127985

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

A375

Predict Reactivity

Mouse, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885672

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3 antibody used in Western Blot (WB). GTX127985

GTX127985 WB Image

Histone H3 antibody detects Histone H3 protein by Western blot analysis.
A. 30 μg A375 whole cell lysate/extract
15 % SDS-PAGE
Histone H3 antibody (GTX127985) dilution: 1:5000

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX127985

GTX127985 IHC-P Image

Histone H3 antibody detects Histone H3 protein at cytosol and nucleus on AGS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded AGS xenograft.
Histone H3 antibody (GTX127985) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3 antibody used in Western Blot (WB). GTX127985

GTX127985 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX127985) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169