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Histone H3 antibody

Anti-Histone H3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130464
Anti-Histone H3 antibody used in Western Blot (WB). GTX130464

Cat. No. GTX130464

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:2000
Not tested in other applications.

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886281

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130464

GTX130464 ICC/IF Image

Histone H3 antibody detects Histone H3 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3 stained by Histone H3 antibody (GTX130464) diluted at 1:2000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Histone H3 antibody used in Western Blot (WB). GTX130464

GTX130464 WB Image

Histone H3 antibody detects Histone H3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX130464) diluted by 1:1000.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169