Histone H3K14ac (Acetyl Lys14) antibody [RM130]

Cat. No. GTX60874

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM130
Isotype	IgG
Application	WB, ICC/IF, ELISA, ChIP assay
Reactivity	Human
Package	100 μg ($449)

See all Histone H3 products

Datasheet

View product citations for antibody GTX60874 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.5 μg/mL - 2 μg/mL
ICC/IF	0.5 μg/mL - 2 μg/mL
ELISA	0.2 μg/mL - 1 μg/mL
ChIP assay	2 μg/mL - 10 μg/mL

Not tested in other applications.

Product Note

This antibody reacts to Histone H3 acetylated at Lysine 14 (K14ac), and is not affected by the modification of neighboring amino acids. No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14)

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60874 Image

The binding specificity of RM130 to Histone H3 acetylated at Lysine 14 (K14ac) is not affected by the modification of neighboring amino acids.

GTX60874 ChIP assay Image

ChIP analysis of HeLa cells using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg

GTX60874 WB Image

WB analysis of acid extracts from HeLa cellsuntreated (-) or treated with sodium butyrate (+) using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130].
Dilution : 0.5μg/ml

GTX60874 Image

The GTX60874 reacts to Histone H3 acetylated at Lysine 14 (K14ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.

GTX60874 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60874 Histone H3K14ac (Acetyl Lys14) antibody [RM130].
Red : Primary antibody
Green : Actin

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 449

Qty

Histone H3K14ac (Acetyl Lys14) antibody [RM130]

Cat. No. GTX60874

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Research Area

DATA IMAGES

GTX60874 Image

GTX60874 ChIP assay Image

GTX60874 WB Image

GTX60874 Image

GTX60874 ICC/IF Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.