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Histone H3K14ac (Acetyl Lys14) antibody

Anti-Histone H3K14ac (Acetyl Lys14) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX88008
Anti-Histone H3K14ac (Acetyl Lys14) antibody used in Western Blot (WB). GTX88008

Cat. No. GTX88008

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μg ($409)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100~1:500
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H3K14ac (6-55).

Purification

Purified by antigen-affinity chromatography. Non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
From serum

Conjugation

Unconjugated

RRID

AB_10731165

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K14ac (Acetyl Lys14) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX88008

GTX88008 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX88008 Histone H3K14ac (Acetyl Lys14) antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Histone H3K14ac (Acetyl Lys14) antibody used in Western Blot (WB). GTX88008

GTX88008 WB Image

The data was published in the journal Mol Oncol in 2019.PMID: 30585695

REFERENCE

Application Reference

Liao L et al. Mol Oncol 2019; 13 (4) : 811-828 High affinity binding of H3K14ac through collaboration of bromodomains 2, 4 and 5 is critical for the molecular and tumor suppressor functions of PBRM1.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409