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Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade

Anti-Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60814
Anti-Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60814
Anti-Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade used in ELISA (ELISA). GTX60814
Anti-Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60814
Anti-Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade used in Dot blot (Dot). GTX60814
Anti-Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade used in Western Blot (WB). GTX60814

Cat. No. GTX60814

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, Dot, ELISA, ChIP assay

Reactivity

 Human, Mouse
Package
50 μg ($429)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:200
Dot 1:5,000
ELISA 1:100
ChIP assay 0.5-5 μg
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region of histone H3 containing the acetylated lysine 18 (H3K18ac), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60814

GTX60814 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells treated with TSA using GTX60814 Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Anti-Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60814

GTX60814 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60814 Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:200

Anti-Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade used in ELISA (ELISA). GTX60814

GTX60814 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60814 Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade.

Anti-Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60814

GTX60814 ChIP assay Image

ChIP analysis of HeLa cells treated with TSA using GTX60814 Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete human X-chromosome and a zoomin to a 600 kb region (figure 2A and B), and in two regions on chromosome 14 and 3 surrounding the c-fos and EIF4A2 positive control genes (figure 2C and D, respectively).
Antibody amount : 1μg

Anti-Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade used in Dot blot (Dot). GTX60814

GTX60814 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3K18 using GTX60814 Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade.
Dilution : 1:5,000

Anti-Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade used in Western Blot (WB). GTX60814

GTX60814 WB Image

WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60814 Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade.
Dilution : 1:500

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Package List Price ($)
$ 429