Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Histone H3K18ac (Acetyl Lys18) antibody [HL1463] detects Histone H3K18ac (Acetyl Lys18) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
Histone H3K18ac (Acetyl Lys18) stained by Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H3K18ac (Acetyl Lys18) antibody [HL1463] detects Histone H3K18ac (Acetyl Lys18) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3K18ac (Acetyl Lys18) stained by Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H3K18ac (Acetyl Lys18) antibody [HL1463] detects Histone H3K18ac (Acetyl Lys18) protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K18ac (Acetyl Lys18) stained by Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Dotblot analysis of anti-Histone H3K18ac (Acetyl Lys18) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3K18ac (Acetyl Lys18) antibody (GTX636937) 1:5000 dilution.
A: Peptide samples of H3K18 un
B: Peptide samples of H3K18ac
C: Peptide samples of H3K9un
D: Peptide samples of H3K9ac
E: Peptide samples of H3K14un
F: Peptide samples of H3K14ac
G: Peptide samples of H3K27un
H: Peptide samples of H3K27ac