APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding acetyl Lys18 of human Histone H3. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
histone cluster 1 H3 family member a , H3/A , H3FA
Cellular Localization
Nucleus
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Database
Research Area
DATA IMAGES
![Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Western Blot (WB). GTX636937](/templates/default/js/lightbox/images/loading.gif "Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Western Blot (WB). GTX636937")
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GTX636937 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636937 IHC-P Image
Histone H3K18ac (Acetyl Lys18) antibody [HL1463] detects Histone H3K18ac (Acetyl Lys18) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
Histone H3K18ac (Acetyl Lys18) stained by Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636937 Dot Image
Dotblot analysis of anti-Histone H3K18ac (Acetyl Lys18) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3K18ac (Acetyl Lys18) antibody (GTX636937) 1:5000 dilution.
A: Peptide samples of H3K18 un
B: Peptide samples of H3K18ac
C: Peptide samples of H3K9un
D: Peptide samples of H3K9ac
E: Peptide samples of H3K14un
F: Peptide samples of H3K14ac
G: Peptide samples of H3K27un
H: Peptide samples of H3K27ac
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GTX636937 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636937 IHC-P Image
Histone H3K18ac (Acetyl Lys18) antibody [HL1463] detects Histone H3K18ac (Acetyl Lys18) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3K18ac (Acetyl Lys18) stained by Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636937 ICC/IF Image
Histone H3K18ac (Acetyl Lys18) antibody [HL1463] detects Histone H3K18ac (Acetyl Lys18) protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H3K18ac (Acetyl Lys18) stained by Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10μm.
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REFERENCE
There are currently no references for Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937). Be the first to share your experience with this product.
![Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Western Blot (WB). GTX636937](/upload/website/prouct_img/small/GTX636937/GTX636937_44725_20220701_WB_treatment_TSA_22070501_366.webp "Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Western Blot (WB). GTX636937")
![Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in IHC (Paraffin sections) (IHC-P). GTX636937](/upload/website/prouct_img/small/GTX636937/GTX636937_T-44662_20220513_IHC-P_R_22053019_784.jpg "Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in IHC (Paraffin sections) (IHC-P). GTX636937")
![Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Dot blot (Dot). GTX636937](/upload/website/prouct_img/small/GTX636937/GTX636937_T-44662_20220610_DOT_22062121_893.webp "Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Dot blot (Dot). GTX636937")
![Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Western Blot (WB). GTX636937](/upload/website/prouct_img/small/GTX636937/GTX636937_T-44662_20220429_WB_treatment_TSA_22050323_556.jpg "Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Western Blot (WB). GTX636937")
![Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in IHC (Paraffin sections) (IHC-P). GTX636937](/upload/website/prouct_img/small/GTX636937/GTX636937_T-44662_20220513_IHC-P_M_22053019_901.jpg "Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in IHC (Paraffin sections) (IHC-P). GTX636937")
![Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636937](/upload/website/prouct_img/small/GTX636937/GTX636937_44725_20221230_ICC_IF_22122901_210.webp "Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636937")
![Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Western Blot (WB). GTX636937](/upload/website/prouct_img/normal/GTX636937/GTX636937_44725_20220701_WB_treatment_TSA_22070501_366.webp "Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Western Blot (WB). GTX636937")
![Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in IHC (Paraffin sections) (IHC-P). GTX636937](/upload/website/prouct_img/normal/GTX636937/GTX636937_T-44662_20220513_IHC-P_R_22053019_784.jpg "Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in IHC (Paraffin sections) (IHC-P). GTX636937")
![Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Dot blot (Dot). GTX636937](/upload/website/prouct_img/normal/GTX636937/GTX636937_T-44662_20220610_DOT_22062121_893.webp "Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Dot blot (Dot). GTX636937")
![Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Western Blot (WB). GTX636937](/upload/website/prouct_img/normal/GTX636937/GTX636937_T-44662_20220429_WB_treatment_TSA_22050323_556.jpg "Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Western Blot (WB). GTX636937")
![Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in IHC (Paraffin sections) (IHC-P). GTX636937](/upload/website/prouct_img/normal/GTX636937/GTX636937_T-44662_20220513_IHC-P_M_22053019_901.jpg "Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in IHC (Paraffin sections) (IHC-P). GTX636937")
![Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636937](/upload/website/prouct_img/normal/GTX636937/GTX636937_44725_20221230_ICC_IF_22122901_210.webp "Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636937")