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Histone H3K18ac (Acetyl Lys18) antibody [HL1463]

Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Dot blot (Dot). GTX636937
Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Western Blot (WB). GTX636937
Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in IHC (Paraffin sections) (IHC-P). GTX636937
Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in IHC (Paraffin sections) (IHC-P). GTX636937
Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636937
Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Western Blot (WB). GTX636937

Cat. No. GTX636937

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1463

Isotype

IgG

Application

WB, ICC/IF, IHC-P, Dot

Reactivity

Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Dot Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys18 of human Histone H3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Dot blot (Dot). GTX636937

GTX636937 Dot Image

Dotblot analysis of anti-Histone H3K18ac (Acetyl Lys18) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3K18ac (Acetyl Lys18) antibody (GTX636937) 1:5000 dilution.
A: Peptide samples of H3K18 un
B: Peptide samples of H3K18ac
C: Peptide samples of H3K9un
D: Peptide samples of H3K9ac
E: Peptide samples of H3K14un
F: Peptide samples of H3K14ac
G: Peptide samples of H3K27un
H: Peptide samples of H3K27ac

Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Western Blot (WB). GTX636937

GTX636937 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in IHC (Paraffin sections) (IHC-P). GTX636937

GTX636937 IHC-P Image

Histone H3K18ac (Acetyl Lys18) antibody [HL1463] detects Histone H3K18ac (Acetyl Lys18) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3K18ac (Acetyl Lys18) stained by Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in IHC (Paraffin sections) (IHC-P). GTX636937

GTX636937 IHC-P Image

Histone H3K18ac (Acetyl Lys18) antibody [HL1463] detects Histone H3K18ac (Acetyl Lys18) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
Histone H3K18ac (Acetyl Lys18) stained by Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636937

GTX636937 ICC/IF Image

Histone H3K18ac (Acetyl Lys18) antibody [HL1463] detects Histone H3K18ac (Acetyl Lys18) protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K18ac (Acetyl Lys18) stained by Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Histone H3K18ac (Acetyl Lys18) antibody [HL1463] used in Western Blot (WB). GTX636937

GTX636937 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody [HL1463] (GTX636937) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations