Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166] (2 ug/mL) as the detection antibody and GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody. Please notice that GTX60886 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166].
Red : Primary antibody
Green : Actin

ChIP analysis of HeLa cells with or without Sodium Butyrate treatment using GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg

ICC/IF analysis of HepG2 cells using GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166].

WB analysis of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) using GTX60886 Histone H3K18ac (Acetyl Lys18) antibody [RM166].
Dilution : 0.5μg/ml

The GTX60886 reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.