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Histone H3K18ac (Acetyl Lys18) antibody

Anti-Histone H3K18ac (Acetyl Lys18) antibody used in ChIP assay (ChIP assay). GTX128943
Anti-Histone H3K18ac (Acetyl Lys18) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128943
Anti-Histone H3K18ac (Acetyl Lys18) antibody used in Western Blot (WB). GTX128943
Anti-Histone H3K18ac (Acetyl Lys18) antibody used in IHC (Paraffin sections) (IHC-P). GTX128943
Anti-Histone H3K18ac (Acetyl Lys18) antibody used in IHC (Paraffin sections) (IHC-P). GTX128943
Anti-Histone H3K18ac (Acetyl Lys18) antibody used in Dot blot (Dot). GTX128943
Anti-Histone H3K18ac (Acetyl Lys18) antibody used in IHC (Paraffin sections) (IHC-P). GTX128943
Anti-Histone H3K18ac (Acetyl Lys18) antibody used in Immunoprecipitation (IP). GTX128943
Anti-Histone H3K18ac (Acetyl Lys18) antibody used in IHC (Paraffin sections) (IHC-P). GTX128943

Cat No. GTX128943

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, Dot, ChIP assay

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Dot Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Positive Control

HeLa

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3K18ac (acetyl Lys18). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Nucleus

Research Area

DATA IMAGES

Anti-Histone H3K18ac (Acetyl Lys18) antibody used in ChIP assay (ChIP assay). GTX128943

GTX128943 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-Histone H3K18ac (acetyl Lys18) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or β-globin promoter.

Anti-Histone H3K18ac (Acetyl Lys18) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128943

GTX128943 ICC/IF Image

Histone H3 (acetyl Lys18) antibody detects Histone H3 (acetyl Lys18) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3 (acetyl Lys18) protein stained by Histone H3 (acetyl Lys18) antibody (GTX128943) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Histone H3K18ac (Acetyl Lys18) antibody used in Western Blot (WB). GTX128943

GTX128943 WB Image

Histone H3 (acetyl Lys18) antibody detects Histone H3 (acetyl Lys18) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 μg HeLa whole cell lysate/extract (0.4 μM Trichostatin A treatment for 18 hr)
15 % SDS-PAGE
Histone H3 (acetyl Lys18) antibody (GTX128943) dilution: 1:5000

Anti-Histone H3K18ac (Acetyl Lys18) antibody used in IHC (Paraffin sections) (IHC-P). GTX128943

GTX128943 IHC-P Image

Histone H3K18ac (acetyl Lys18) antibody detects Histone H3K18ac (acetyl Lys18) protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Histone H3K18ac (acetyl Lys18) antibody (GTX128943) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K18ac (Acetyl Lys18) antibody used in IHC (Paraffin sections) (IHC-P). GTX128943

GTX128943 IHC-P Image

Histone H3K18ac (acetyl Lys18) antibody detects Histone H3K18ac (acetyl Lys18) protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Histone H3K18ac (acetyl Lys18) antibody (GTX128943) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K18ac (Acetyl Lys18) antibody used in Dot blot (Dot). GTX128943

GTX128943 Dot Image

Dotblot analysis of anti-Histone H3 (acetyl Lys18) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys18) antibody (GTX128943) 1:5000 dilution.
A: Peptide samples of H3K18 un
B: Peptide samples of H3K18ac
C: Peptide samples of H3K9un
D: Peptide samples of H3K9ac
E: Peptide samples of H3K14un
F: Peptide samples of H3K14ac
G: Peptide samples of H3K27un
H: Peptide samples of H3K27ac

Anti-Histone H3K18ac (Acetyl Lys18) antibody used in IHC (Paraffin sections) (IHC-P). GTX128943

GTX128943 IHC-P Image

Histone H3K18ac (acetyl Lys18) antibody detects Histone H3K18ac (acetyl Lys18) protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3K18ac (acetyl Lys18) antibody (GTX128943) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K18ac (Acetyl Lys18) antibody used in Immunoprecipitation (IP). GTX128943

GTX128943 IP Image

Histone H3 (acetyl Lys18) antibody immunoprecipitates Histone H3 (acetyl Lys18) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H3 (acetyl Lys18) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H3 (acetyl Lys18) protein by 2.5 μg of Histone H3 (acetyl Lys18) antibody (GTX128943) 12% SDS-PAGE The immunoprecipitated Histone H3 protein (acetyl Lys18) was detected by Histone H3 (acetyl Lys18) antibody (GTX128943) diluted at 1 : 5000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Histone H3K18ac (Acetyl Lys18) antibody used in IHC (Paraffin sections) (IHC-P). GTX128943

GTX128943 IHC-P Image

Histone H3K18ac (acetyl Lys18) antibody detects Histone H3K18ac (acetyl Lys18) protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Histone H3K18ac (acetyl Lys18) antibody (GTX128943) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169