GeneTex
United States (US)

Histone H3K23ac (Acetyl Lys23) antibody

Histone-H3-acetyl-Lys23-antibody-GTX88017-WB-1_18122102_743.jpg
Histone-H3-acetyl-Lys23-antibody-GTX88017-ICC_IF-1_18122102_222.jpg

Cat No. GTX88017

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:1000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

15 kDa. ( Note )

Specificity/Sensitivity

Histone H3 (Acetyl-Lys23) Antibody detects endogenous levels of total Histone H3 protein only when acetylated at lysine23.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys23.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acet

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H3/j, HIST1H3A, H3/d, histone H3.1, H3/k, H3/c, H3/i, Histone H3, H3/f, H31, H3/a, H3/l, H3/h, Histone H3 (acetyl K23), Histone H3 (acetyl Lys23), Histone H3 acetyl K23, Histone H3 acetyl Lys23, Acetyl Histone H3 (K23), Acetyl Histone H3 (Lys23)

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Research Area

DATA IMAGES
Histone-H3-acetyl-Lys23-antibody-GTX88017-WB-1_18122102_743.jpg

GTX88017 WB Image

Western blot analysis of extracts from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-Lys23) Antibody. The lane on the right is treated with the synthesized peptide.

Histone-H3-acetyl-Lys23-antibody-GTX88017-ICC_IF-1_18122102_222.jpg

GTX88017 ICC/IF Image

Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys23) Antibody. The picture on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339