Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade

ChIP analysis of sheared chromatin from 1x10⁵ K562 cells using GTX60815 Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2A shows the peak distribution along the complete human X-chromosome. Figure 2 B and C show the peak distribution in two regions surrounding the EIF4A2 and GAPDH positive control genes, respectively. The position of the PCR amplicon, used for validating the ChIP assay is indicated with an arrow.
Antibody amount : 1μg

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3K27 using GTX60815 Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade.
Dilution : 1:20,000

ELISA analysis of peptide containing the histone modification of interest using GTX60815 Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade.

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60815 Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade. A titration consisting of 0.2, 0.5, 1 and 2 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as positive controls, and for the coding regions of the inactive MB and MYT1 genes, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60815 Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60815 Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade.
Dilution : 1:1,000