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Histone H3K27ac (Acetyl Lys27) antibody [GT261]

Orthogonal
Anti-Histone H3K27ac (Acetyl Lys27) antibody [GT261] used in Western Blot (WB). GTX633652
Anti-Histone H3K27ac (Acetyl Lys27) antibody [GT261] used in IHC (Paraffin sections) (IHC-P). GTX633652
Anti-Histone H3K27ac (Acetyl Lys27) antibody [GT261] used in IHC (Paraffin sections) (IHC-P). GTX633652

Cat. No. GTX633652

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT261

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

HeLa(0.4 μM Trichostatin A treatment for 18 hr)

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys27 of human Histone H3. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888358

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K27ac (Acetyl Lys27) antibody [GT261] used in Western Blot (WB). GTX633652

GTX633652 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (acetyl Lys27) antibody [GT261] (GTX633652) diluted at 1:1000.

Anti-Histone H3K27ac (Acetyl Lys27) antibody [GT261] used in IHC (Paraffin sections) (IHC-P). GTX633652

GTX633652 IHC-P Image

Histone H3K27ac (acetyl Lys27) antibody [GT261] detects Histone H3K27ac (acetyl Lys27) protein at nucleus in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Histone H3K27ac (acetyl Lys27) antibody [GT261] (GTX633652) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3K27ac (Acetyl Lys27) antibody [GT261] used in IHC (Paraffin sections) (IHC-P). GTX633652

GTX633652 IHC-P Image

Histone H3K27ac (acetyl Lys27) antibody [GT261] detects Histone H3K27ac (acetyl Lys27) protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Histone H3K27ac (acetyl Lys27) antibody [GT261] (GTX633652) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations