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Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade

Anti-Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60350
Anti-Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60350
Anti-Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade used in ELISA (ELISA). GTX60350
Anti-Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade used in Dot blot (Dot). GTX60350
Anti-Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade used in Western Blot (WB). GTX60350

Cat No. GTX60350

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, Dot, ELISA, ChIP assay

Reactivity

Human, Arabidopsis thaliana
Package
50 μg ($389)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
Dot Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Histone H3 containing the monomethylated lysine 27 (H3K27me1), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60350

GTX60350 ChIP assay Image

ChIP analysis of sheared chromatin from 5x10⁵ K562 cells using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Figure 1A. Quantitative PCR was performed with primers for the coding sequence of the active GAPDH and ACTB genes, used as positive controls, and for the inactive MYOD1 and MYT1 genes, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H3K27me1, H3K27me2, H3K27me3, H3K4me1, H3K9me1, H3K36me1, H4K20me1 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is specific in ChIP for the H3K27me1 modification with some slight cross reaction with H3K36me1 and H3K9me1 at higher concentrations.

Anti-Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60350

GTX60350 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed U2OS cells using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade. Staining of the cells with the H3K27me1 antibody after incubation of the antibody with 2 ng/μl blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K27 (Figure B, C, D, respectively).
Red : Primary antibody
Blue : DAPI
Dilution : 1:1000

Anti-Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade used in ELISA (ELISA). GTX60350

GTX60350 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade.

Anti-Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade used in Dot blot (Dot). GTX60350

GTX60350 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications and unmodified sequences of histone H3 and H4 using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade.
Dilution : 1:20,000

Anti-Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade used in Western Blot (WB). GTX60350

GTX60350 WB Image

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade.
Dilution : 1:1,000

REFERENCE

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Package List Price ($)
$ 389