Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade

Cat. No. GTX60350

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, Dot, ELISA, ChIP assay
Reactivity	Human, Arabidopsis thaliana
Package	50 μg ($429)

See all Histone H3 products

Datasheet

View product citations for antibody GTX60350 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
Dot	Assay dependent
ELISA	Assay dependent
ChIP assay	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Histone H3 containing the monomethylated lysine 27 (H3K27me1), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60350 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade.

GTX60350 ChIP assay Image

ChIP analysis of sheared chromatin from 5x10⁵ K562 cells using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Figure 1A. Quantitative PCR was performed with primers for the coding sequence of the active GAPDH and ACTB genes, used as positive controls, and for the inactive MYOD1 and MYT1 genes, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H3K27me1, H3K27me2, H3K27me3, H3K4me1, H3K9me1, H3K36me1, H4K20me1 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is specific in ChIP for the H3K27me1 modification with some slight cross reaction with H3K36me1 and H3K9me1 at higher concentrations.

GTX60350 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications and unmodified sequences of histone H3 and H4 using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade.
Dilution : 1:20,000

GTX60350 WB Image

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade.
Dilution : 1:1,000

GTX60350 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed U2OS cells using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade. Staining of the cells with the H3K27me1 antibody after incubation of the antibody with 2 ng/μl blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K27 (Figure B, C, D, respectively).
Red : Primary antibody
Blue : DAPI
Dilution : 1:1000

REFERENCE

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REVIEW

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Package

List Price ($)

50 μg

$ 429

Qty

Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade

Cat. No. GTX60350

Host

Clonality

Isotype

Application

Reactivity