Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade

ICC/IF analysis of 4% paraformaldehyde fixed U2OS cells using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade. Staining of the cells with the H3K27me1 antibody after incubation of the antibody with 2 ng/μl blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K27 (Figure B, C, D, respectively).
Red : Primary antibody
Blue : DAPI
Dilution : 1:1000

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade.
Dilution : 1:1,000

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications and unmodified sequences of histone H3 and H4 using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade.
Dilution : 1:20,000

ChIP analysis of sheared chromatin from 5x10⁵ K562 cells using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Figure 1A. Quantitative PCR was performed with primers for the coding sequence of the active GAPDH and ACTB genes, used as positive controls, and for the inactive MYOD1 and MYT1 genes, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H3K27me1, H3K27me2, H3K27me3, H3K4me1, H3K9me1, H3K36me1, H4K20me1 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is specific in ChIP for the H3K27me1 modification with some slight cross reaction with H3K36me1 and H3K9me1 at higher concentrations.

ELISA analysis of peptide containing the histone modification of interest using GTX60350 Histone H3K27me1 (Mono-methyl Lys27) antibody - ChIP grade.