GeneTex
  • Country / Location Selection

United States (US)

Histone H3K27me1 (mono-methyl Lys27) antibody

Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in ChIP assay (ChIP assay). GTX54104
Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54104
Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in Dot blot (Dot). GTX54104
Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in Western Blot (WB). GTX54104
Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in Western Blot (WB). GTX54104
Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54104
Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54104
Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54104

Cat No. GTX54104

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, Dot, ChIP assay

Reactivity

Human, Mouse, Rat
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Dot Assay dependent
ChIP assay 1:20 - 1:100
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone cluster 3 H3 , H3.4 , H3/g , H3FT , H3t

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in ChIP assay (ChIP assay). GTX54104

GTX54104 ChIP assay Image

ChIP analysis of 293T cell lysate using GTX54104 Histone H3K27me1 (mono-methyl Lys27) antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54104

GTX54104 ICC/IF Image

ICC/IF analysis of 293T cells using GTX54104 Histone H3K27me1 (mono-methyl Lys27) antibody.
Blue : DAPI

Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in Dot blot (Dot). GTX54104

GTX54104 Dot Image

Dot blot analysis of all sorts of methylation peptides using GTX54104 Histone H3K27me1 (mono-methyl Lys27) antibody.

Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in Western Blot (WB). GTX54104

GTX54104 WB Image

WB analysis of various sample lysates using GTX54104 Histone H3K27me1 (mono-methyl Lys27) antibody.
Loading : 25μg per lane

Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in Western Blot (WB). GTX54104

GTX54104 WB Image

WB analysis of various sample lysates using GTX54104 Histone H3K27me1 (mono-methyl Lys27) antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54104

GTX54104 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX54104 Histone H3K27me1 (mono-methyl Lys27) antibody.
Dilution : 1:100

Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54104

GTX54104 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX54104 Histone H3K27me1 (mono-methyl Lys27) antibody.
Dilution : 1:100

Anti-Histone H3K27me1 (mono-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54104

GTX54104 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX54104 Histone H3K27me1 (mono-methyl Lys27) antibody.
Dilution : 1:100

REFERENCE

There are currently no references for Histone H3K27me1 (mono-methyl Lys27) antibody (GTX54104). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Histone H3K27me1 (mono-methyl Lys27) antibody (GTX54104). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379