Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP grade

Cat. No. GTX60351

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, Dot, ELISA, ChIP assay
Reactivity	Human, Zebrafish
Package	50 μg ($429)

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Datasheet

View product citations for antibody GTX60351 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
Dot	Assay dependent
ELISA	Assay dependent
ChIP assay	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The histone H3, dimethylated at lysine 27 (H3K27me2), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60351 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60351 Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

GTX60351 WB Image

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60351 Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP grade.
Dilution : 1:1,000

GTX60351 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60351 Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the promoter of the inactive HBB and the coding region of the inactive MYOD1 genes, used as positive controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me2 is preferably present at silent genes.

GTX60351 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60351 Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP grade.

GTX60351 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 and H4 and the unmodified sequence using GTX60351 Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP grade.
Dilution : 1:50,000

REFERENCE

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Package

List Price ($)

50 μg

$ 429

Qty

Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP grade

Cat. No. GTX60351

Host

Clonality

Isotype

Application

Reactivity