Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 and H4 and the unmodified sequence using GTX60351 Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP grade.
Dilution : 1:50,000

ELISA analysis of peptide containing the histone modification of interest using GTX60351 Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP grade.

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60351 Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the promoter of the inactive HBB and the coding region of the inactive MYOD1 genes, used as positive controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K27me2 is preferably present at silent genes.

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60351 Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP grade.
Dilution : 1:1,000

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60351 Histone H3K27me2 (Di-methyl Lys27) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500