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Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60816
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in Dot blot (Dot). GTX60816
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60816
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in ELISA (ELISA). GTX60816
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in Western Blot (WB). GTX60816
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60816

Cat. No. GTX60816

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, Dot, ELISA, ChIP assay

Reactivity

 Human, Mouse, Drosophila, Arabidopsis thaliana, Caenorhabditis elegans, Zea mays, Tomato, Poplar, Daphnia, Cyanidioschyzon merolae
Package
50 μg ($529)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:200
Dot 1:5,000
ELISA 1:100 - 1:500
ChIP assay 0.5-5 μg
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region of histone H3 containing the trimethylated lysine 27 (H3K27me3), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60816

GTX60816 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60816 Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment in genomic regions of chromosome 6 and 20, surrounding the TSH2B and MYT1 positive control genes (fig 2A and 2B, respectively), and in two genomic regions of chromosome 1 and X (figure 2C and D).
Antibody amount : 1μg

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in Dot blot (Dot). GTX60816

GTX60816 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 and H4 and the unmodified H3K27 sequence using GTX60816 Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade.
Dilution : 1:5,000

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60816

GTX60816 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60816 Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:200

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in ELISA (ELISA). GTX60816

GTX60816 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60816 Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in Western Blot (WB). GTX60816

GTX60816 WB Image

WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells and 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) proteins using GTX60816 Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade.
Dilution : 1:500

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60816

GTX60816 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60816 Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade. A titration consisting of 0.5, 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Figure 1A. Quantitative PCR was performed with primers specific for the promoter of the active GAPDH and EIF4A2 genes, used as negative controls, and for the inactive TSH2B and MYT1 genes, used as positive controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H3K27me1, H3K27me2, H3K27me3, H3K4me3, H3K9me3 and H3K36me3 modifications and the unmodified H3K27 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K27me3 modification.

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REVIEW

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Package List Price ($)
$ 529