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Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60816
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in ELISA (ELISA). GTX60816
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60816
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in Dot blot (Dot). GTX60816
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in Western Blot (WB). GTX60816

Cat No. GTX60816

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, Dot, ELISA, ChIP assay, ChIP-seq

Reactivity

Human, Mouse
Package
50 μg ($459)

APPLICATION

Application Note

ChIP (1 μg/IP), ELISA (1:1,000), Dot blotting/Peptide array (1:20,000), Western blotting (1:1,000)

PROPERTIES

Form

Liquid

Buffer

PBS containing 0.05% azide and 0.05% ProClin 300.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.90 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Polyclonal antibody raised in rabbit against the region of histone H3 containing the trimethylated lysine 27 (H3K27me3), using a KLH-conjugated synthetic peptide.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60816

GTX60816 ChIP assay Image

ChIP was performed with K562 extracts and either control IgG (1 μg) or Histone H3K27me3 (tri-Methy Lys27) antibody (0.1, 0.5, and 1 μg per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to positive controls MYT1 and TSH2B genes, and to negative controls GAPDH and EIF4A2 promoters.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in ELISA (ELISA). GTX60816

GTX60816 ELISA Image

ELISA was performed using a serial dilution of Histone H3K27me3 (tri-Methy Lys27) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:22,400.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60816

GTX60816 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using H3K27me3 antibody (green) and DAPI (blue). Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with H3K27me3 antibody at a dilution of 1:500.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in Dot blot (Dot). GTX60816

GTX60816 Dot Image

Dot blot analysis of peptides containing modifications or unmodified sequences of histone H3 and H4 using H3K27me3 antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody - ChIP grade used in Western Blot (WB). GTX60816

GTX60816 WB Image

WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using H3K27me3 antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.

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SDS
PBS.pdf
Proclin.pdf
Package List Price ($)
$ 459