GeneTex
  • Country / Location Selection

United States (US)

Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175]

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175] used in Western Blot (WB). GTX60892
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175] used in ELISA (ELISA). GTX60892

Cat No. GTX60892

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM175

Isotype

IgG

Application

WB, ICC/IF, ELISA, IHC, Multiplexing

Reactivity

Human, Mouse
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
IHC Assay dependent
Multiplexing Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This antibody reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3.

PROPERTIES

Form

Liquid

Buffer

PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys27).

Purification

Protein A affinity purified from an animal origin–free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H3/A Antibody , HIST1H3A Antibody , H3FA Antibody , histone cluster 1, H3a Antibody , H3K27Me3 antibody

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]

Research Area

DATA IMAGES

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175] used in Western Blot (WB). GTX60892

GTX60892 WB Image

Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM175 at 1 ug/mL, showed a band of histone H3 trimethylated at Lysine 27 (K27me3) in HeLa cells.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175] used in ELISA (ELISA). GTX60892

GTX60892 ELISA Image

RM175 specifically reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with non-modified Lysine 27 (K27 Ctrl), monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2),or other methylations in Histone H3.

REFERENCE

REVIEW

There are currently no reviews for Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175] (GTX60892). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399