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Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175]

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60892
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175] used in Western Blot (WB). GTX60892
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175] used in  (). GTX60892

Cat No. GTX60892

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM175

Isotype

IgG

Application

WB, ICC/IF, ELISA, ChIP assay, IHC

Reactivity

Human, Mouse
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 μg/mL - 2 μg/mL
ICC/IF Assay dependent
ELISA 0.5μg/mL - 1 μg/mL
ChIP assay Assay dependent
IHC 0.5 μg/mL - 2 μg/mL
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Product Note

This antibody reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity non-modified Lysine 27, monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 0.09% sodium azide, 50% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys27).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888006

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Nucleus,Chromosome

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60892

GTX60892 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60892 Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175].

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175] used in Western Blot (WB). GTX60892

GTX60892 WB Image

WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60892 Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175].
Dilution : 1μg/ml

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody [RM175] used in  (). GTX60892

GTX60892 Image

The GTX60892 reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with non-modified Lysine 27 (K27 Ctrl), monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2),or other methylations in Histone H3.

REFERENCE

REVIEW

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