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GeneTex
United States (US)

Histone H3K27me3 (Tri-methyl Lys27) antibody

Orthogonal
GTX121184_40506_20150320_IHC_M_2_18121410_718.jpg
GTX121184_41983_20161229_WB_M_18121410_878.jpg
GTX121184_40800_20150226_WB_18121410_832.jpg
GTX121184_40359_IFA_18121410_386.jpg
GTX121184_40506_20150814_IHC-Fr_M_18121410_157.jpg
GTX121184_40800_20170425_IHC-Wm_Z_18121410_294.jpg
GTX121184_41983_20150518_IHC-P_M_2_18121410_663.jpg
GTX121184_40359_IHC_18121410_126.jpg
GTX121184_40506_WB_18121410_958.jpg
GTX121184_40800_20160114_DOT_18121410_684.jpg
GTX121184_40359_CT_WB_18121410_253.jpg

Cat No. GTX121184

Reference 5
3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, Dot, EM
Reactivity Human, Mouse, Rat, Zebrafish
See all Histone H3 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC-Wm 1:100-1:500
Dot Assay dependent
EM Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Jurkat , Raji , K562 , THP-1 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3K27me3 (trimethyl Lys27). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Nucleus

Research Area

DATA IMAGES
GTX121184_40506_20150320_IHC_M_2_18121410_718.jpg

GTX121184 IHC-P Image

Histone H3K27me3 (trimethyl Lys27) antibody detects Histone H3K27me3 (trimethyl Lys27) protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX121184_41983_20161229_WB_M_18121410_878.jpg

GTX121184 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:1000.
GTX121184_40800_20150226_WB_18121410_832.jpg

GTX121184 WB Image

Histone H3 (tri-Methyl Lys27) antibody detects Histone H3 (tri-Methyl Lys27) protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys27) antibody (GTX121184) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX121184_40359_IFA_18121410_386.jpg

GTX121184 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using Histone H3 (tri-Methyl K27)(GTX121184) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
GTX121184_40506_20150814_IHC-Fr_M_18121410_157.jpg

GTX121184 IHC-Fr Image

Immunofluorescence photomicrographs of frozen sections of mouse liver.
Red: Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight594) (GTX213110-05).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX121184_40800_20170425_IHC-Wm_Z_18121410_294.jpg

GTX121184 IHC-Wm Image

Histone H3K27me3 (trimethyl Lys27) antibody detects Histone H3K27me3 (trimethyl Lys27) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) dilution: 1:100.
GTX121184_41983_20150518_IHC-P_M_2_18121410_663.jpg

GTX121184 IHC-P Image

Histone H3K27me3 (trimethyl Lys27) antibody detects Histone H3K27me3 (trimethyl Lys27) protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3K27me3 (trimethyl Lys27) antibody (GTX121184) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX121184_40359_IHC_18121410_126.jpg

GTX121184 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histon H3 (tri-Methyl K27)(GTX121184) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX121184_40506_WB_18121410_958.jpg

GTX121184 WB Image

Sample (30 µg of whole cell lysate)
A: Jurkat
B: Raji
C: K562
D: THP-1
7.5% SDS PAGE
GTX121184 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX121184_40800_20160114_DOT_18121410_684.jpg

GTX121184 Dot Image

Various peptide samples were spotted onto positively charged nylon membrane, and blotted with Histone H3 (tri-Methyl Lys27) antibody (GTX121184) diluted at 1:2500.
GTX121184_40359_CT_WB_18121410_253.jpg

GTX121184 WB Image

U87 cells were grown under normoxic (DMEM 10% FBS, 16% O2), hypoxic (DMEM 10% FBS, 1% O2), and neurosphere conditions (DMEM/F12, B-27 supplement, growth factor (10ng/ml FGF and 20ng/ml EGF)). Cell lysate were Western blotted for H3K27me3 and Histone-H3 (loading control). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
REFERENCE

Application Reference

Tsuyama N et al. Hematol Oncol 2017; Epstein-Barr virus-negative extranodal "true" natural killer-cell lymphoma harbouring a KDM6A mutation.
Application : IHC-P
Reactivity : Human
Mirzamohammadi F et al. Nat Commun 2016; 7 : 12047 Polycomb repressive complex 2 regulates skeletal growth by suppressing Wnt and TGF-β signalling.
Application : WB
Reactivity : Mouse
Perrigue PM et al. Mol Cancer Res 2015; 13 (4) : 636-50 The histone demethylase jumonji coordinates cellular senescence including secretion of neural stem cell-attracting cytokines.
Liu TP et al. Anticancer Drugs 2015; 26 (2) : 139-47 S-Adenosyl-L-methionine-competitive inhibitors of the histone methyltransferase EZH2 induce autophagy and enhance drug sensitivity in cancer cells.
Application : WB
Reactivity : Human
Jiang X et al. FASEB J 2012; 26 (8) : 3563-74 Maternal choline intake alters the epigenetic state of fetal cortisol-regulating genes in humans.
Submit a Reference
REVIEW

Histone H3K27me3 (Tri-methyl Lys27) antibody Cat No. GTX121184

Rating
( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4 based on 2 users reviews)
Electron microscopy(EM)
( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, overnightHr
Lane Description : Lane 1: MG63 whole cell lysate, Lane 2: doxorubicin resistant MG63 cell line, Lane 3: Cisplatin resistant MG63 cell line
Date : Anonymous submitted on 25-Jul-2016
Score :
Application Tested : EM
Sample Species : Rat
Sample : liver tissue
Blocking : Normal Goat Serum in PBS, 25°C, 0.08Hr
Fixation : 4% PFA in S?rensen phosphate buffer (pH 7.2)
Permeabilization /Antigen retrieval : 0.1% BSA in PBST
Primary Antibody : 1:50, 4°C, overnightHr
Date : Anonymous submitted on 20-Jan-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 60μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169