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Histone H3K27me3 (Tri-methyl Lys27) antibody

Orthogonal
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX129774
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX129774
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX129774
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX129774
Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129774

Cat. No. GTX129774

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

HeLa nuclear extract , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys27 of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886084

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX129774

GTX129774 WB Image

Orthogonal

Histone H3 (tri-Methyl Lys27) antibody detects Histone H3 (tri-Methyl Lys27) protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys27) antibody (GTX129774) diluted by 1:1000.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX129774

GTX129774 IHC-P Image

Histone H3 (tri-Methyl Lys27) antibody detects Histone H3 (tri-Methyl Lys27) protein at nucleus on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H3 (tri-Methyl Lys27) antibody (GTX129774) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX129774

GTX129774 IHC-P Image

Histone H3 (tri-Methyl Lys27) antibody detects Histone H3 (tri-Methyl Lys27) protein at nucleus on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H3 (tri-Methyl Lys27) antibody (GTX129774) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Western Blot (WB). GTX129774

GTX129774 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (tri-Methyl Lys27) antibody (GTX129774) diluted at 1:1000.

Anti-Histone H3K27me3 (Tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129774

GTX129774 ICC/IF Image

Histone H3 (tri-Methyl Lys27) antibody detects Histone H3 (tri-Methyl Lys27) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3 (tri-Methyl Lys27) protein stained by Histone H3 (tri-Methyl Lys27) antibody (GTX129774) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169