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Histone H3K27me3 (tri-methyl Lys27) antibody

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Dot blot (Dot). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in ChIP assay (ChIP assay). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Western Blot (WB). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106

Cat No. GTX54106

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, Dot, ChIP assay

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Dot Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2631277

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Dot blot (Dot). GTX54106

GTX54106 Dot Image

Dot blot analysis of all sorts of methylation peptides using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in ChIP assay (ChIP assay). GTX54106

GTX54106 ChIP assay Image

ChIP analysis of 293T cell lysate using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Western Blot (WB). GTX54106

GTX54106 WB Image

WB analysis of various sample lysates using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106

GTX54106 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106

GTX54106 ICC/IF Image

ICC/IF analysis of C6 cells using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106

GTX54106 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106

GTX54106 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106

GTX54106 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106

GTX54106 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf