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Histone H3K27me3 (tri-methyl Lys27) antibody

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Western Blot (WB). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Dot blot (Dot). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in ChIP assay (ChIP assay). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106
Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106

Cat No. GTX54106

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, Dot, ChIP assay

Reactivity

Human, Mouse, Rat, Pig, Rice, Cotton, Zea mays
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
IP 1:50-1:200
Dot Assay dependent
ChIP assay 1:20-1:100
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106

GTX54106 IHC-P Image

IHC-P analysis of rat heart tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106

GTX54106 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Western Blot (WB). GTX54106

GTX54106 WB Image

WB analysis of various sample lysates using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106

GTX54106 IHC-P Image

IHC-P analysis of human stomach tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106

GTX54106 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106

GTX54106 ICC/IF Image

ICC/IF analysis of C6 cells using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106

GTX54106 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Dot blot (Dot). GTX54106

GTX54106 Dot Image

Dot blot analysis of various methylation peptides using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in ChIP assay (ChIP assay). GTX54106

GTX54106 ChIP assay Image

ChIP analysis of 293T cells using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106

GTX54106 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54106

GTX54106 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3K27me3 (tri-methyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX54106

GTX54106 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319