GeneTex
United States (US)

Histone H3K27me3 (tri-methyl Lys27) antibody

GTX54106_20180530_IHC-P_3_18121410_166.jpg
GTX54106_20180530_IHC-P_1_18121410_960.jpg
GTX54106_20180530_ChIPassay_18121410_543.jpg
GTX54106_20180530_IHC-P_18121410_299.jpg
GTX54106_20180530_Dot_18121410_998.jpg
GTX54106_20180530_ICCIF_1_18121410_168.jpg
GTX54106_20180530_IHC-P_2_18121410_577.jpg
GTX54106_20180530_WB_18121410_505.jpg
GTX54106_20180530_ICCIF_18121410_535.jpg

Cat No. GTX54106

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, Dot, ChIP assay
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Dot Assay dependent
ChIP assay 1:20 - 1:100
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX54106_20180530_IHC-P_3_18121410_166.jpg

GTX54106 IHC-P Image

IHC-P analysis of rat testis tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:200

GTX54106_20180530_IHC-P_1_18121410_960.jpg

GTX54106 IHC-P Image

IHC-P analysis of human thyroid cancer tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:200

GTX54106_20180530_ChIPassay_18121410_543.jpg

GTX54106 ChIP assay Image

ChIP analysis of 293T cells using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

GTX54106_20180530_IHC-P_18121410_299.jpg

GTX54106 IHC-P Image

IHC-P analysis of human rectal cancer tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:200

GTX54106_20180530_Dot_18121410_998.jpg

GTX54106 Dot Image

Dot blot analysis of all sorts of methylation peptides using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.

GTX54106_20180530_ICCIF_1_18121410_168.jpg

GTX54106 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Red : Primary antibody
Green : BrdU antibody
Blue : DAPI

GTX54106_20180530_IHC-P_2_18121410_577.jpg

GTX54106 IHC-P Image

IHC-P analysis of rat brain tissue using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:200

GTX54106_20180530_WB_18121410_505.jpg

GTX54106 WB Image

WB analysis of various samples using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX54106_20180530_ICCIF_18121410_535.jpg

GTX54106 ICC/IF Image

ICC/IF analysis of 293T cell using GTX54106 Histone H3K27me3 (tri-methyl Lys27) antibody.
Blue : DAPI

Package List Price ($)
$ 319