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Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade

Anti-Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade used in ELISA (ELISA). GTX60329
Anti-Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade used in Immunoprecipitation (IP). GTX60329
Anti-Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade used in Western Blot (WB). GTX60329
Anti-Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade used in Dot blot (Dot). GTX60329
Anti-Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60329
Anti-Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60329

Cat. No. GTX60329

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, Dot, ELISA, ChIP assay

Reactivity

 Human
Package
50 μl ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:250 - 1:500
ICC/IF 1:200
IP 5 μl
Dot 1:20,000
ELISA 1:100 - 1:500
ChIP assay 1 μl
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Histone H3 containing the trimethylated lysine 27 and the phosphorylated serine 28 (H3K27me3S28p), using a KLH-conjugated synthetic peptide.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade used in ELISA (ELISA). GTX60329

GTX60329 ELISA Image

ELISA analysis of peptide containing the histone modifications of interest using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade.

Anti-Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade used in Immunoprecipitation (IP). GTX60329

GTX60329 IP Image

IP analysis of HeLa cells were treated with colcemid (to block the cell cycle in metaphase) using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade.
IP reaction : 5 μl antibody / 1x10⁴ cells
Lane 1 : Primary antibody
Lane 2 : No antibody control
Lane 3 : Input
Dilution : 1:500

Anti-Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade used in Western Blot (WB). GTX60329

GTX60329 WB Image

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade.
Dilution : 1:250

Anti-Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade used in Dot blot (Dot). GTX60329

GTX60329 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 and H4 and unmodified sequences from histone H3 using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade.
Dilution : 1:20,000

Anti-Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60329

GTX60329 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁴ HeLa cells treated with colcemid using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade. A titration of the antibody consisting of 1, 5 and 10 μl per ChIP experiment was analysed. Additionally, ChIP was performed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and RPL30 and for the inactive gene MYOD. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Anti-Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60329

GTX60329 ICC/IF Image

ICC/IF analysis of paraformaldehyde fixed HeLa asynchronous cells using GTX60329 Histone H3K27me3S28ph (Tri-methyl Lys27/phospho Ser28) antibody - ChIP grade. Phosphorylation of H3 on serine 28 is increased during late G2 phase and reaches a maximum in metaphase cells. This may explain the different staining intensities of different cells.
Green : Primary antibody
Blue : DAPI
Dilution : 1:200

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Package List Price ($)
$ 399