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Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade

Anti-Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60372
Anti-Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade used in ELISA (ELISA). GTX60372
Anti-Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60372
Anti-Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade used in Western Blot (WB). GTX60372
Anti-Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade used in Dot blot (Dot). GTX60372
Anti-Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60372

Cat. No. GTX60372

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, Dot, ELISA, ChIP assay

Reactivity

 Human, Mouse, Yeast
Package
50 μl ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:500
Dot 1:100,000
ELISA 1:1,000
ChIP assay 0.5-10 μl
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Histone H3 containing the dimethylated lysine 36 (H3K36me2), using a KLH-conjugated synthetic peptide.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60372

GTX60372 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁴ HeLa cells using GTX60372 Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade. A titration of the antibody consisting of 1, 5, and 10 μl per ChIP experiment was analysed. Additionally, the same titration was analysed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes GAPDH and ALDOA and for the coding region of the myogenic differentiation gene (MYOD). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Anti-Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade used in ELISA (ELISA). GTX60372

GTX60372 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60372 Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade.

Anti-Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60372

GTX60372 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60372 Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

Anti-Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade used in Western Blot (WB). GTX60372

GTX60372 WB Image

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60372 Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade.
Dilution : 1:1,000

Anti-Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade used in Dot blot (Dot). GTX60372

GTX60372 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications and unmodified sequences of histone H3 using GTX60372 Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade.
Dilution : 1:100,000

Anti-Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60372

GTX60372 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60372 Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the signal distribution along 3 genomic regions of chromosome 20, 12 and X, respectively.
Antibody amount : 0.5μl

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Package List Price ($)
$ 399