Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60372 Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the signal distribution along 3 genomic regions of chromosome 20, 12 and X, respectively.
Antibody amount : 0.5μl

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications and unmodified sequences of histone H3 using GTX60372 Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade.
Dilution : 1:100,000

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60372 Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade.
Dilution : 1:1,000

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60372 Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

ELISA analysis of peptide containing the histone modification of interest using GTX60372 Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade.

ChIP analysis of sheared chromatin from 10⁴ HeLa cells using GTX60372 Histone H3K36me2 (Di-methyl Lys36) antibody - ChIP grade. A titration of the antibody consisting of 1, 5, and 10 μl per ChIP experiment was analysed. Additionally, the same titration was analysed after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the promoter of the active genes GAPDH and ALDOA and for the coding region of the myogenic differentiation gene (MYOD). This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).