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Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade

Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60818
Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in Protein Array (Protein Array). GTX60818
Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in Western Blot (WB). GTX60818
Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60818
Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60818
Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in Dot blot (Dot). GTX60818
Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in ELISA (ELISA). GTX60818
Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60818

Cat. No. GTX60818

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, Dot, ELISA, ChIP assay, Protein Array

Reactivity

 Human, Mouse
Package
50 μg ($529)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:200
Dot 1:5,000/1:2,000
ELISA 1:400
ChIP assay 0.5-5 μg
Protein Array Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region of histone H3 containing the monomethylated lysine 4 (H3K4me1), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2888005

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60818

GTX60818 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60818 Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:200

Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in Protein Array (Protein Array). GTX60818

GTX60818 Protein Array Image

Protein Array analysis of an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B using GTX60818 Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade. This figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.
Dilution : 1:2,000

Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in Western Blot (WB). GTX60818

GTX60818 WB Image

WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60818 Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade.
Dilution : 1:500

Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60818

GTX60818 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ K562 cells using GTX60818 Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for a region surrounding the ACTB and GAS2L1 gene, respectively, used as positive controls, and for the promoters of the GAPDH and EIF4A2 genes, used as negative controls. Figure 2 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60818

GTX60818 ChIP assay Image

ChIP analysis of sheared chromatin from 1x10⁵ K562 cells using GTX60818 Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 3A and B show the H3K4me1 signal in two genomic regions containing the ACTB and GAS2L1 positive controls. The position of the amplicon used for ChIP-qPCR is indicated by an arrow. Figure 3C shows the H3K4me1 peak distribution along a 1 Mb genomic region of chromosome 5.
Antibody amount : 1μg

Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in Dot blot (Dot). GTX60818

GTX60818 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3K4 using GTX60818 Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade.
Dilution : 1:5,000

Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in ELISA (ELISA). GTX60818

GTX60818 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60818 Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade.

Anti-Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60818

GTX60818 ChIP assay Image

ChIP analysis of sheared chromatin from 5x10⁵ HeLaS3 cells using GTX60818 Histone H3K4me1 (Mono-methyl Lys4) antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Figure 1A. Quantitative PCR was performed with primers for a region surrounding the ACTB and GAS2L1 genes, used as positive controls, and for the promoters of the GAPDH and EIF4A2 genes, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H3K4me1, H3K4me2, H3K4me3, H3K9me1, H3K27me1, H3K36me1, H4K20me1 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K4me1 modification.

REFERENCE

Application Reference

Eid W et al. Biol Chem 2016; (Epub) Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A.
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Package List Price ($)
$ 529