ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140].
Red : Primary antibody
Green : Actin

WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140].
Dilution : 0.5μg/ml

ChIP analysis of HeLa cells using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg

ELISA analysis of various methylated Histone H3 peptides using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140].
It specifically reacts to Histone H3 monomethylated at Lysine 4 (K4me1) and no cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in Histone H3.