Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]

Cat. No. GTX60878

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM140
Isotype	IgG
Application	WB, ICC/IF, ELISA, ChIP assay
Reactivity	Human
Package	100 μg ($449)

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Datasheet

View product citations for antibody GTX60878 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.2 μg/mL - 1 μg/mL
ICC/IF	1 μg/mL - 2 μg/mL
ELISA	0.2 μg/mL - 1 μg/mL
ChIP assay	2 μg/mL - 10 μg/mL

Not tested in other applications.

Product Note

This antibody reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys4)

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60878 ChIP assay Image

ChIP analysis of HeLa cells using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg

GTX60878 WB Image

WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140].
Dilution : 0.5μg/ml

GTX60878 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140].
Red : Primary antibody
Green : Actin

GTX60878 ELISA Image

ELISA analysis of various methylated Histone H3 peptides using GTX60878 Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140].
It specifically reacts to Histone H3 monomethylated at Lysine 4 (K4me1) and no cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in Histone H3.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 449

Qty

Histone H3K4me1 (Mono-methyl Lys4) antibody [RM140]

Cat. No. GTX60878

Host

Clonality

Clone Name

Isotype

Application

Reactivity