Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60819 Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade.
Dilution : 1:1,000

ELISA analysis of peptide containing the histone modification of interest using GTX60819 Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade.

ChIP analysis of sheared chromatin from 5x10⁵ HeLaS3 cells using GTX60819 Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Figure 1A. Quantitative PCR was performed with primers for a region upstream of the ACTB and GAS2L1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H3K4me1, H3K4me2, H3K4me3, H3K9me2, H3K27me2, H3K36me2, H4K20me2 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K4me2 modification.

ICC/IF analysis of 4% paraformaldehyde fixed U2OS cells using GTX60819 Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade. Staining of the cells with the H3K4me2 antibody after incubation of the antibody with 5 ng/μl blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K4 (Figure B, C, D, respectively).
Red : Primary antibody
Blue : DAPI
Dilution : 1:5000

ChIP analysis of sheared chromatin from 5x10⁵ HeLaS3 cells using GTX60819 Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade. The IP'd DNA was analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete X-chromosome (figure 2A) and in 3 chromosomal regions surrounding the GAPDH, c-fos and ACTB genes (figure 2B, C and D, respectively).
Antibody amount : 1μg

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 and H4 and the unmodified H3K4 sequence using GTX60819 Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade.
Dilution : 1:20,000