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Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade used in Dot blot (Dot). GTX60819
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60819
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60819
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60819
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade used in ELISA (ELISA). GTX60819
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade used in Western Blot (WB). GTX60819

Cat. No. GTX60819

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, Dot, ELISA, ChIP assay

Reactivity

 Human, Arabidopsis thaliana
Package
50 μg ($429)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:5,000
Dot 1:20,000
ELISA 1:500
ChIP assay 0.5-5 μg
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Histone H3 containing the dimethylated lysine 4 (H3K4me2), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade used in Dot blot (Dot). GTX60819

GTX60819 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 and H4 and the unmodified H3K4 sequence using GTX60819 Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade.
Dilution : 1:20,000

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60819

GTX60819 ChIP assay Image

ChIP analysis of sheared chromatin from 5x10⁵ HeLaS3 cells using GTX60819 Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade. The IP'd DNA was analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete X-chromosome (figure 2A) and in 3 chromosomal regions surrounding the GAPDH, c-fos and ACTB genes (figure 2B, C and D, respectively).
Antibody amount : 1μg

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60819

GTX60819 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed U2OS cells using GTX60819 Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade. Staining of the cells with the H3K4me2 antibody after incubation of the antibody with 5 ng/μl blocking peptide containing the unmodified and the mono-, di- and trimethylated H3K4 (Figure B, C, D, respectively).
Red : Primary antibody
Blue : DAPI
Dilution : 1:5000

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60819

GTX60819 ChIP assay Image

ChIP analysis of sheared chromatin from 5x10⁵ HeLaS3 cells using GTX60819 Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade. A titration of the antibody consisting of 0.5, 1, 2 and 5 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Figure 1A. Quantitative PCR was performed with primers for a region upstream of the ACTB and GAS2L1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The graph shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1B. Recovery of the nucleosomes carrying the H3K4me1, H3K4me2, H3K4me3, H3K9me2, H3K27me2, H3K36me2, H4K20me2 and the unmodified H3K4 as determined by qPCR. The figure clearly shows the antibody is very specific in ChIP for the H3K4me2 modification.

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade used in ELISA (ELISA). GTX60819

GTX60819 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60819 Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade.

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade used in Western Blot (WB). GTX60819

GTX60819 WB Image

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60819 Histone H3K4me2 (Di-methyl Lys4) antibody - ChIP grade.
Dilution : 1:1,000

REFERENCE

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Package List Price ($)
$ 429