Histone H3K4me2 (Di-methyl Lys4) antibody [RM135]

Cat. No. GTX60875

Host	Rabbit
Clonality	Monoclonal
Clone Name	RM135
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human
Package	100 μg ($449)

See all Histone H3 products

Datasheet

View product citations for antibody GTX60875 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.02 μg/mL - 0.2 μg/mL
ICC/IF	1 μg/mL - 2 μg/mL
ELISA	0.2 μg/mL - 1 μg/mL

Not tested in other applications.

Product Note

This antibody reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys4).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60875 WB Image

WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60875 Histone H3K4me2 (Di-methyl Lys4) antibody [RM135].
Dilution : 0.025μg/ml

GTX60875 Image

The GTX60875 reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with mono- methylated Lysine 4 (K4me1), trimethylated Lysine 4(K4me3), or other methylations in histone H3.

GTX60875 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60875 Histone H3K4me2 (Di-methyl Lys4) antibody [RM135].
Red : Primary antibody
Green : Actin

REFERENCE

There are currently no references for Histone H3K4me2 (Di-methyl Lys4) antibody [RM135] (GTX60875). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for Histone H3K4me2 (Di-methyl Lys4) antibody [RM135] (GTX60875). Be the first to share your experience with this product.

Submit a Review

Package

List Price ($)

100 μg

$ 449

Qty

Histone H3K4me2 (Di-methyl Lys4) antibody [RM135]

Cat. No. GTX60875

Host

Clonality

Clone Name

Isotype

Application

Reactivity