Histone H3 (di-methyl Lys4) antibody immunoprecipitates Histone H3 (di-methyl Lys4) protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Histone H3 (di-methyl Lys4) protein by 2 μg of Histone H3 (di-methyl Lys4) antibody (GTX121915) 15% SDS-PAGE The immunoprecipitated Histone H3 (di-methyl Lys4) protein was detected by Histone H3 (di-methyl Lys4) antibody (GTX121915) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me2 (Di-methyl Lys4) antibody (GTX121915) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3K4me2 (dimethyl Lys4) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or β-globin promoter.

Histone H3K4me2 (dimethyl Lys4) antibody detects Histone H3K4me2 (dimethyl Lys4) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K4me2 (dimethyl Lys4) protein stained by Histone H3K4me2 (dimethyl Lys4) antibody (GTX121915) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

Histone H3K4me2 (dimethyl Lys4) antibody detects Histone H3K4me2 (dimethyl Lys4) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K4me2 (dimethyl Lys4) protein stained by Histone H3K4me2 (dimethyl Lys4) antibody (GTX121915) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Histone H3 (di-Methyl K4)(GTX121915) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min