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Histone H3K4me2 (Di-methyl Lys4) antibody

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Immunoprecipitation (IP). GTX121915
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX121915
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Western Blot (WB). GTX121915
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121915
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121915
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in ChIP assay (ChIP assay). GTX121915

Cat No. GTX121915

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3K4me2 (dimethyl Lys4). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Nucleus

Research Area

DATA IMAGES
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Immunoprecipitation (IP). GTX121915

GTX121915 IP Image

Histone H3 (di-methyl Lys4) antibody immunoprecipitates Histone H3 (di-methyl Lys4) protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Histone H3 (di-methyl Lys4) protein by 2 μg of Histone H3 (di-methyl Lys4) antibody (GTX121915) 15% SDS-PAGE The immunoprecipitated Histone H3 (di-methyl Lys4) protein was detected by Histone H3 (di-methyl Lys4) antibody (GTX121915) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX121915

GTX121915 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Histone H3 (di-Methyl K4)(GTX121915) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Western Blot (WB). GTX121915

GTX121915 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me2 (dimethyl Lys4) antibody (GTX121915) diluted at 1:5000.

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121915

GTX121915 ICC/IF Image

Histone H3K4me2 (dimethyl Lys4) antibody detects Histone H3K4me2 (dimethyl Lys4) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K4me2 (dimethyl Lys4) protein stained by Histone H3K4me2 (dimethyl Lys4) antibody (GTX121915) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121915

GTX121915 ICC/IF Image

Histone H3K4me2 (dimethyl Lys4) antibody detects Histone H3K4me2 (dimethyl Lys4) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K4me2 (dimethyl Lys4) protein stained by Histone H3K4me2 (dimethyl Lys4) antibody (GTX121915) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in ChIP assay (ChIP assay). GTX121915

GTX121915 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3K4me2 (dimethyl Lys4) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or β-globin promoter.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169