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Histone H3K4me2 (Di-methyl Lys4) antibody

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Immunoprecipitation (IP). GTX121915
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX121915
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Western Blot (WB). GTX121915
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121915
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121915
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in ChIP assay (ChIP assay). GTX121915

Cat. No. GTX121915

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding di-methyl Lys4 of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722383

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Immunoprecipitation (IP). GTX121915

GTX121915 IP Image

Histone H3 (di-methyl Lys4) antibody immunoprecipitates Histone H3 (di-methyl Lys4) protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Histone H3 (di-methyl Lys4) protein by 2 μg of Histone H3 (di-methyl Lys4) antibody (GTX121915) 15% SDS-PAGE The immunoprecipitated Histone H3 (di-methyl Lys4) protein was detected by Histone H3 (di-methyl Lys4) antibody (GTX121915) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX121915

GTX121915 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Histone H3 (di-Methyl K4)(GTX121915) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Western Blot (WB). GTX121915

GTX121915 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me2 (dimethyl Lys4) antibody (GTX121915) diluted at 1:5000.

Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121915

GTX121915 ICC/IF Image

Histone H3K4me2 (dimethyl Lys4) antibody detects Histone H3K4me2 (dimethyl Lys4) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K4me2 (dimethyl Lys4) protein stained by Histone H3K4me2 (dimethyl Lys4) antibody (GTX121915) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121915

GTX121915 ICC/IF Image

Histone H3K4me2 (dimethyl Lys4) antibody detects Histone H3K4me2 (dimethyl Lys4) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K4me2 (dimethyl Lys4) protein stained by Histone H3K4me2 (dimethyl Lys4) antibody (GTX121915) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Anti-Histone H3K4me2 (Di-methyl Lys4) antibody used in ChIP assay (ChIP assay). GTX121915

GTX121915 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3K4me2 (dimethyl Lys4) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or β-globin promoter.

REFERENCE

Application Reference

Pasternak T et al. Planta 2023; 258 (4) : 76 Regulation of early seedling establishment and root development in Arabidopsis thaliana by light and carbohydrates.
Eid W et al. Biol Chem 2016; (Epub) Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A.
Jiang X et al. FASEB J 2012; 26 (8) : 3563-74 Maternal choline intake alters the epigenetic state of fetal cortisol-regulating genes in humans.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169