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Histone H3K4me2 (di-methyl Lys4) antibody

Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX55660
Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in ChIP assay (ChIP assay). GTX55660
Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in Dot blot (Dot). GTX55660
Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55660
Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in Western Blot (WB). GTX55660
Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in Western Blot (WB). GTX55660
Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX55660
Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX55660
Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55660

Cat No. GTX55660

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, Dot, ChIP assay

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Dot Assay dependent
ChIP assay 1:50 - 1:200
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

histone cluster 3 H3 , H3.4 , H3/g , H3FT , H3t

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Database

Research Area

DATA IMAGES

Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX55660

GTX55660 IHC-P Image

IHC-P analysis of rat brain tissue using GTX55660 Histone H3K4me2 (di-methyl Lys4) antibody.
Dilution : 1:100

Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in ChIP assay (ChIP assay). GTX55660

GTX55660 ChIP assay Image

ChIP analysis of 293 cell lysate using GTX55660 Histone H3K4me2 (di-methyl Lys4) antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in Dot blot (Dot). GTX55660

GTX55660 Dot Image

Dot blot analysis of all sorts of methylation peptides using GTX55660 Histone H3K4me2 (di-methyl Lys4) antibody.

Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55660

GTX55660 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX55660 Histone H3K4me2 (di-methyl Lys4) antibody.
Blue : DAPI
Dilution : 1:100

Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in Western Blot (WB). GTX55660

GTX55660 WB Image

WB analysis of various sample lysates using GTX55660 Histone H3K4me2 (di-methyl Lys4) antibody.
Loading : 25μg per lane

Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in Western Blot (WB). GTX55660

GTX55660 WB Image

WB analysis of various sample lysates using GTX55660 Histone H3K4me2 (di-methyl Lys4) antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX55660

GTX55660 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX55660 Histone H3K4me2 (di-methyl Lys4) antibody.
Dilution : 1:200

Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX55660

GTX55660 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX55660 Histone H3K4me2 (di-methyl Lys4) antibody.
Dilution : 1:100

Anti-Histone H3K4me2 (di-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55660

GTX55660 ICC/IF Image

ICC/IF analysis of 293T cells using GTX55660 Histone H3K4me2 (di-methyl Lys4) antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf