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Histone H3K4me3 (Tri-methyl Lys4) antibody

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in ChIP assay (ChIP assay). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Western Blot (WB). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Western Blot (WB). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Dot blot (Dot). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954
Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Whole mount) (IHC-Wm). GTX128954

Cat No. GTX128954

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Wm, Dot, ChIP assay, EM

Reactivity

Human, Mouse, Rat, Zebrafish
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm 1:100-1:500
Dot Assay dependent
ChIP assay Assay dependent
EM Assay dependent
Not tested in other applications.

Positive Control

HeLa nuclear , whole zebrafish

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Human Histone H3K4me3 (Tri-methyl Lys4). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Nucleus

Research Area

DATA IMAGES

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954

GTX128954 IHC-P Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954

GTX128954 IHC-P Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in ChIP assay (ChIP assay). GTX128954

GTX128954 ChIP assay Image

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-Histone H3K4me3 (trimethyl Lys4) antibody. The precipitated DNA was detected by PCR with primer set targeting to GADPH gene.

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Western Blot (WB). GTX128954

GTX128954 WB Image

Whole zebrafish extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Western Blot (WB). GTX128954

GTX128954 WB Image

Histone H3 (tri-Methyl Lys4) antibody detects Histone H3 (tri-Methyl Lys4) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
15 % SDS-PAGE
Histone H3 (tri-Methyl Lys4) antibody (GTX128954) dilution: 1:1000

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Dot blot (Dot). GTX128954

GTX128954 Dot Image

Dotblot analysis of anti-Histone H3 (tri-Methyl Lys4) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (tri-Methyl Lys4) antibody (GTX128954) 1:5000 dilution.
A: Peptide samples of H3K4 un
B: Peptide samples of H3K4me1
C: Peptide samples of H3K4me2
D: Peptide samples of H3K4me3
E: Peptide samples of H3K4ac

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128954

GTX128954 ICC/IF Image

Histone H3 (tri-Methyl Lys4) antibody detects Histone H3 (tri-Methyl Lys4) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3 (tri-Methyl Lys4) protein stained by Histone H3 (tri-Methyl Lys4) antibody (GTX128954) diluted at 1:500.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954

GTX128954 IHC-P Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954

GTX128954 IHC-P Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Whole mount) (IHC-Wm). GTX128954

GTX128954 IHC-Wm Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:100.

REFERENCE

REVIEW

Histone H3K4me3 (Tri-methyl Lys4) antibody Cat No. GTX128954

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Electron microscopy(EM) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, 16Hr
Lane Description : Lane 1: MG63 whole cell lysate, Lane 2: doxorubicin resistant MG63 cell line, Lane 3: Cisplatin resistant MG63 cell line
Date : Anonymous submitted on 27-Mar-2017
Score :
Application Tested : EM
Sample Species : Ms
Sample : liver tissue
Blocking : Normal Goat Serum in PBS, 25°C, 0.08Hr
Fixation : 4% PFA in S?rensen phosphate buffer (pH 7.2)
Permeabilization /Antigen retrieval : 0.1% BSA in PBST
Primary Antibody : 1:10, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169