Histone H3K4me3 (Tri-methyl Lys4) antibody

Name: Histone H3K4me3 (Tri-methyl Lys4) antibody
Brand: GeneTex
SKU: GTX128954
Price: 399.00 USD
Rating: 4 (2 reviews)

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Paraffin sections) (IHC-P). GTX128954

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in ChIP assay (ChIP assay). GTX128954

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Western Blot (WB). GTX128954

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Dot blot (Dot). GTX128954

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in IHC (Whole mount) (IHC-Wm). GTX128954

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128954

Cat. No. GTX128954

Reference 8

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, Dot, ChIP assay, EM
Reactivity	Human, Mouse, Rat, Zebrafish, Drosophila
Package	100 μl ($399), 25 μl ($169)

See all Histone H3 products

View product citations for antibody GTX128954 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent
IHC-Wm	1:100-1:500
Dot	Assay dependent
ChIP assay	Assay dependent
EM	Assay dependent

Not tested in other applications.

Positive Control

whole zebrafish

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys4 of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2783841

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX128954 IHC-P Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128954 IHC-P Image

Histone H3K4me3 (Tri-methyl Lys4) antibody detects Histone H3K4me3 (Tri-methyl Lys4) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3K4me3 (Tri-methyl Lys4) stained by Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128954 IHC-P Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128954 IHC-P Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128954 IHC-P Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX128954 ChIP assay Image

ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-Histone H3K4me3 (trimethyl Lys4) antibody. The precipitated DNA was detected by PCR with primer set targeting to GADPH gene.

GTX128954 WB Image

Whole zebrafish extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX128954 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX128954 Dot Image

Dotblot analysis of anti-Histone H3 (tri-Methyl Lys4) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (tri-Methyl Lys4) antibody (GTX128954) 1:5000 dilution.
A: Peptide samples of H3K4 un
B: Peptide samples of H3K4me1
C: Peptide samples of H3K4me2
D: Peptide samples of H3K4me3
E: Peptide samples of H3K4ac

GTX128954 IHC-Wm Image

Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:100.

GTX128954 ICC/IF Image

Histone H3 (tri-Methyl Lys4) antibody detects Histone H3 (tri-Methyl Lys4) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3 (tri-Methyl Lys4) protein stained by Histone H3 (tri-Methyl Lys4) antibody (GTX128954) diluted at 1:500.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX128954 IHC-P Image

Histone H3K4me3 (Tri-methyl Lys4) antibody detects Histone H3K4me3 (Tri-methyl Lys4) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Histone H3K4me3 (Tri-methyl Lys4) stained by Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX128954 ICC/IF Image

Histone H3K4me3 (Tri-methyl Lys4) antibody detects Histone H3K4me3 (Tri-methyl Lys4) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K4me3 (Tri-methyl Lys4) stained by Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX128954 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Shangguan H et al. G3 (Bethesda) 2024; 14 (3) Knockdown of Kmt2d leads to growth impairment by activating the Akt/β-catenin signaling pathway.

Pang L et al. Biosci Rep 2021; 41 (11) KMT2D deficiency disturbs the proliferation and cell cycle activity of dental epithelial cell line (LS8) partially via Wnt signaling.

Application : IHC-Fr

Reactivity : Mouse

Chiu YF et al. Sci Rep 2020; 10 (1) : 8261 Critical role of SOX2-IGF2 signaling in aggressiveness of bladder cancer.

Di Giorgio E et al. Nucleic Acids Res 2020; 48 (2) : 646-664 Different class IIa HDACs repressive complexes regulate specific epigenetic responses related to cell survival in leiomyosarcoma cells.

Application : WB

Laurent C et al. Blood 2019; (Epub) Gene alterations in epigenetic modifiers and JAK-STAT signaling are frequent in breast implant-associated ALCL.

Show More +

Submit a Reference

REVIEW

Histone H3K4me3 (Tri-methyl Lys4) antibody Cat. No. GTX128954

Rating

( Average 4 based on 2 users reviews)

Application

Western Blot(WB)		( Average 5 based on 1 users reviews)
Electron microscopy(EM)		( Average 3 based on 1 users reviews)

Date : Anonymous submitted on 26-May-2018

Score :

Application Tested : WB

Sample Species : Hu

Sample : MG63 cells

Amount used : 30 μg

Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr

Primary Antibody : 1:100, 4°C, 16Hr

Lane Description : Lane 1: MG63 whole cell lysate, Lane 2: doxorubicin resistant MG63 cell line, Lane 3: Cisplatin resistant MG63 cell line

Anti-Histone H3K4me3 (Tri-methyl Lys4) antibody used in Electron microscopy (EM). GTX128954

Date : Anonymous submitted on 27-Mar-2017

Score :

Application Tested : EM

Sample Species : Ms

Sample : liver tissue

Blocking : Normal Goat Serum in PBS, 25°C, 0.08Hr

Fixation : 4% PFA in S?rensen phosphate buffer (pH 7.2)

Permeabilization /Antigen retrieval : 0.1% BSA in PBST

Primary Antibody : 1:10, 4°C, 16Hr

Submit a Review

Application Reference

Shangguan H et al. G3 (Bethesda) 2024; 14 (3) Knockdown of Kmt2d leads to growth impairment by activating the Akt/β-catenin signaling pathway.

Pang L et al. Biosci Rep 2021; 41 (11) KMT2D deficiency disturbs the proliferation and cell cycle activity of dental epithelial cell line (LS8) partially via Wnt signaling.

Application : IHC-Fr

Reactivity : Mouse

Chiu YF et al. Sci Rep 2020; 10 (1):8261 Critical role of SOX2-IGF2 signaling in aggressiveness of bladder cancer.

Di Giorgio E et al. Nucleic Acids Res 2020; 48 (2):646-664 Different class IIa HDACs repressive complexes regulate specific epigenetic responses related to cell survival in leiomyosarcoma cells.

Application : WB

Laurent C et al. Blood 2019; (Epub) Gene alterations in epigenetic modifiers and JAK-STAT signaling are frequent in breast implant-associated ALCL.

Meng M et al. Immunogenetics 2019; 71 (7):489-499 Methylation of H3K27 and H3K4 in key gene promoter regions of thymus in RA mice is involved in the abnormal development and differentiation of iNKT cells.

Di Giorgio E et al. PLoS Genet 2017; 13 (4):e1006752 The co-existence of transcriptional activator and transcriptional repressor MEF2 complexes influences tumor aggressiveness.

Application : ChIP assay

Reactivity : Human

Eid W et al. Biol Chem 2016; (Epub) Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty

Histone H3K4me3 (Tri-methyl Lys4) antibody

Cat. No. GTX128954

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Research Area

DATA IMAGES

GTX128954 IHC-P Image

GTX128954 IHC-P Image

GTX128954 IHC-P Image

GTX128954 IHC-P Image

GTX128954 IHC-P Image

GTX128954 ChIP assay Image

GTX128954 WB Image

GTX128954 WB Image

GTX128954 Dot Image

GTX128954 IHC-Wm Image

GTX128954 ICC/IF Image

GTX128954 IHC-P Image

GTX128954 ICC/IF Image

GTX128954 WB Image

REFERENCE

Application Reference

REVIEW

Histone H3K4me3 (Tri-methyl Lys4) antibody Cat. No. GTX128954

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.