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Histone H3K56ac (Acetyl Lys56) antibody [RM179]

Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Western Blot (WB). GTX60902
Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60902
Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60902
Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in  (). GTX60902
Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Western Blot (WB). GTX60902
Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Western Blot (WB). GTX60902
Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Western Blot (WB). GTX60902
Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Western Blot (WB). GTX60902
Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Western Blot (WB). GTX60902

Cat. No. GTX60902

Host

Rabbit

Clonality

Monoclonal

Clone Name

RM179

Isotype

IgG

Application

WB, ICC/IF, ELISA, ChIP assay, IHC

Reactivity

Human
Package
100 μg ($449)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL - 2 μg/mL
ICC/IF 0.5 μg/mL - 2 μg/mL
ELISA 0.5 μg/mL - 1 μg/mL
ChIP assay Assay dependent
IHC 1 μg/mL - 10 μg/mL
Not tested in other applications.

Product Note

This antibody reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with other acetylated Lysines in histone H3.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys56).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888008

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Western Blot (WB). GTX60902

GTX60902 WB Image

WB analysis of extracts of HeLa cells treated (Lane 1) with sodium butyrate and recombinant Histone H3.3 protein (Lane 2) using GTX60902 Histone H3K56ac (Acetyl Lys56) antibody [RM179].

Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60902

GTX60902 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60902 Histone H3K56ac (Acetyl Lys56) antibody [RM179].
Red : Primary antibody
Green : Actin

Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60902

GTX60902 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60902 Histone H3K56ac (Acetyl Lys56) antibody [RM179].

Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in  (). GTX60902

GTX60902 Image

The GTX60902 reacts to Histone H3 acetylated at Lysine 56(K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl),acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac),Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.

Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Western Blot (WB). GTX60902

GTX60902 WB Image

The data was published in the journal Oncotarget in 2017.PMID: 29262658

Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Western Blot (WB). GTX60902

GTX60902 WB Image

The data was published in the journal Oncotarget in 2017.PMID: 29262658

Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Western Blot (WB). GTX60902

GTX60902 WB Image

The data was published in the journal Oncotarget in 2017.PMID: 29262658

Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Western Blot (WB). GTX60902

GTX60902 WB Image

The data was published in the journal Oncotarget in 2017.PMID: 29262658

Anti-Histone H3K56ac (Acetyl Lys56) antibody [RM179] used in Western Blot (WB). GTX60902

GTX60902 WB Image

The data was published in the journal Oncotarget in 2017.PMID: 29262658

REFERENCE

REVIEW

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Package List Price ($)
$ 449