WB analysis of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) using GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156].
Dilution : 1μg/ml

The GTX60883 reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetyl- ated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14(K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), or Lysine 122 (K122) in Histone H3.

Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156] (5 ug/mL) as the capture antibody and biotinylated GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the detection antibody. Please notice that GTX60888 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60883. Biotin-conjugated form is not provided.

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156].
Red : Primary antibody
Green : Actin

ChIP analysis of HeLa cells with or without Sodium Butyrate treatment using GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg

Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the detection antibody and GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156] (5 ug/mL) as the capture antibody. Please notice that GTX60888 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60883. Biotin-conjugated form is not provided.