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Histone H3K79ac (Acetyl Lys79) antibody [RM156]

Orthogonal
Anti-Histone H3K79ac (Acetyl Lys79) antibody [RM156] used in ELISA (ELISA). GTX60883
Anti-Histone H3K79ac (Acetyl Lys79) antibody [RM156] used in ChIP assay (ChIP assay). GTX60883
Anti-Histone H3K79ac (Acetyl Lys79) antibody [RM156] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60883
Anti-Histone H3K79ac (Acetyl Lys79) antibody [RM156] used in ELISA (ELISA). GTX60883
Anti-Histone H3K79ac (Acetyl Lys79) antibody [RM156] used in (). GTX60883
Anti-Histone H3K79ac (Acetyl Lys79) antibody [RM156] used in Western Blot (WB). GTX60883

Cat. No. GTX60883

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 RM156

Isotype

 IgG

Application

 WB, ICC/IF, ELISA, ChIP assay, Sandwich ELISA

Reactivity

 Human
Package
100 μg ($459)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 μg/mL - 2 μg/mL
ICC/IF 0.5 μg/mL - 2 μg/mL
ELISA 0.2 μg/mL - 1 μg/mL
ChIP assay 2 μg/mL - 10 μg/mL
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX60883, Detection : GTX60888. Please notice that GTX60888 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60883. Biotin-conjugated form is not provided.
Not tested in other applications.

Product Note

This antibody reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with other acetylated Lysines in Histone H3.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys79).

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K79ac (Acetyl Lys79) antibody [RM156] used in ELISA (ELISA). GTX60883

GTX60883 ELISA Image

Orthogonal

Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the detection antibody and GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156] (5 ug/mL) as the capture antibody. Please notice that GTX60888 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60883. Biotin-conjugated form is not provided.

Anti-Histone H3K79ac (Acetyl Lys79) antibody [RM156] used in ChIP assay (ChIP assay). GTX60883

GTX60883 ChIP assay Image

Orthogonal

ChIP analysis of HeLa cells with or without Sodium Butyrate treatment using GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg

Anti-Histone H3K79ac (Acetyl Lys79) antibody [RM156] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60883

GTX60883 ICC/IF Image

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156].
Red : Primary antibody
Green : Actin

Anti-Histone H3K79ac (Acetyl Lys79) antibody [RM156] used in ELISA (ELISA). GTX60883

GTX60883 ELISA Image

Orthogonal

Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156] (5 ug/mL) as the capture antibody and biotinylated GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the detection antibody. Please notice that GTX60888 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60883. Biotin-conjugated form is not provided.

Anti-Histone H3K79ac (Acetyl Lys79) antibody [RM156] used in (). GTX60883

GTX60883 Image

The GTX60883 reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetyl- ated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14(K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), or Lysine 122 (K122) in Histone H3.

Anti-Histone H3K79ac (Acetyl Lys79) antibody [RM156] used in Western Blot (WB). GTX60883

GTX60883 WB Image

Orthogonal

WB analysis of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) using GTX60883 Histone H3K79ac (Acetyl Lys79) antibody [RM156].
Dilution : 1μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 459