Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for a region 1 kb upstream of the promoter and the coding region of the active GAPDH gene, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

ELISA analysis of peptide containing the histone modification of interest using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade.

ChIP analysis of HeLa cells using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence and a 1 Mb region of the X-chromosome (figure 2A and B), in 100 kb regions surrounding the GAPDH positive control and EIF4A2 genes (figure 2C and D).
Antibody amount : 1μg

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3K79 using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade.
Dilution : 1:20,000

WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H3 (lane 3) using GTX60353 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade.
Dilution : 1:200