GeneTex

  • Country / Location Selection

United States (US)

Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade

Anti-Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60821
Anti-Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade used in Western Blot (WB). GTX60821
Anti-Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade used in Dot blot (Dot). GTX60821
Anti-Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade used in ELISA (ELISA). GTX60821

Cat. No. GTX60821

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, Dot, ELISA, ChIP assay

Reactivity

 Human
Package
50 μl ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
Dot 1:100,000
ELISA 1:500 - 1:1,000
ChIP assay 5 – 10 μl
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Histone H3 the monomethylated lysine 79 (H3K79me1), using a KLH-conjugated synthetic peptide.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60821

GTX60821 ChIP assay Image

ChIP analysis of sheared chromatin from 5x10⁶HeLa cells using GTX60821 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade. IgG (5 μg/IP) was used as a negative IP control. The IP'd DNA was analysed by qPCR using primers for different positive and negative loci. The results are expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1A: recovery of the GAPDH promoter and myoglobin exon 2 with a titration of the H3K79me1 antibody consisting of 5, 10 and 15 μl per ChIP experiment. Figure 1B: recovery of RPL30, ALDOA, SERPINA1 and SAA4 using 10 μl of antibody per ChIP experiment.

Anti-Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade used in Western Blot (WB). GTX60821

GTX60821 WB Image

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60821 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade.
Dilution : 1:1,000 and 1:2000

Anti-Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade used in Dot blot (Dot). GTX60821

GTX60821 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H3 using GTX60821 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade.
Dilution : 1:100,000

Anti-Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade used in ELISA (ELISA). GTX60821

GTX60821 ELISA Image

ELISA analysis of peptides containing the histone modification of interest using GTX60821 Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade.

REFERENCE

There are currently no references for Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade (GTX60821). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Histone H3K79me1 (Mono-methyl Lys79) antibody - ChIP grade (GTX60821). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399