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Histone H3K79me2 (Di-methyl Lys79) antibody

Anti-Histone H3K79me2 (Di-methyl Lys79) antibody used in Dot blot (Dot). GTX60330
Anti-Histone H3K79me2 (Di-methyl Lys79) antibody used in ChIP assay (ChIP assay). GTX60330
Anti-Histone H3K79me2 (Di-methyl Lys79) antibody used in ELISA (ELISA). GTX60330
Anti-Histone H3K79me2 (Di-methyl Lys79) antibody used in Western Blot (WB). GTX60330
Anti-Histone H3K79me2 (Di-methyl Lys79) antibody used in ChIP assay (ChIP assay). GTX60330

Cat. No. GTX60330

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, Dot, ELISA, ChIP assay

Reactivity

 Human
Package
50 μg ($429)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200
Dot 1:5,000
ELISA 1:500
ChIP assay 1-2 μg
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region of histone H3 containing the dimethylated lysine 79 (H3K79me2), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K79me2 (Di-methyl Lys79) antibody used in Dot blot (Dot). GTX60330

GTX60330 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications and unmodified sequences of histone H3 using GTX60330 Histone H3K79me2 (Di-methyl Lys79) antibody.
Dilution : 1:5,000

Anti-Histone H3K79me2 (Di-methyl Lys79) antibody used in ChIP assay (ChIP assay). GTX60330

GTX60330 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60330 Histone H3K79me2 (Di-methyl Lys79) antibody. A titration consisting of 0.5, 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the coding regions of the active EIF2S3 and CCT5 genes, used as positive controls, and for the inactive MYOD1) gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Anti-Histone H3K79me2 (Di-methyl Lys79) antibody used in ELISA (ELISA). GTX60330

GTX60330 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60330 Histone H3K79me2 (Di-methyl Lys79) antibody.

Anti-Histone H3K79me2 (Di-methyl Lys79) antibody used in Western Blot (WB). GTX60330

GTX60330 WB Image

WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60330 Histone H3K79me2 (Di-methyl Lys79) antibody.
Dilution : 1:200

Anti-Histone H3K79me2 (Di-methyl Lys79) antibody used in ChIP assay (ChIP assay). GTX60330

GTX60330 ChIP assay Image

ChIP analysis of HeLa cells using GTX60330 Histone H3K79me2 (Di-methyl Lys79) antibody. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 5 Mb region of chromosome 1 (figure 2A and B) and in two 300 kb regions surrounding the EIF2S3 and CCT5 positive control genes (figure 2C and D).
Antibody amount : 1μg

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Package List Price ($)
$ 429