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Histone H3K79me2 (di-methyl Lys79) antibody

Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in Immunoprecipitation (IP). GTX54110
Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in Western Blot (WB). GTX54110
Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in IHC (Paraffin sections) (IHC-P). GTX54110
Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in IHC (Paraffin sections) (IHC-P). GTX54110
Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in ChIP assay (ChIP assay). GTX54110
Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54110
Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in Dot blot (Dot). GTX54110
Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in IHC (Paraffin sections) (IHC-P). GTX54110

Cat. No. GTX54110

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, Dot, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:100
IP 1:50 - 1:200
Dot Assay dependent
ChIP assay 1:50 - 1:200
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human DiMethyl-Histone H3-K79

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in Immunoprecipitation (IP). GTX54110

GTX54110 IP Image

IP analysis of HeLa cell lysate using GTX54110 Histone H3K79me2 (di-methyl Lys79) antibody.
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in Western Blot (WB). GTX54110

GTX54110 WB Image

WB analysis of HeLa cell lysate using GTX54110 Histone H3K79me2 (di-methyl Lys79) antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in IHC (Paraffin sections) (IHC-P). GTX54110

GTX54110 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX54110 Histone H3K79me2 (di-methyl Lys79) antibody.
Dilution : 1:200

Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in IHC (Paraffin sections) (IHC-P). GTX54110

GTX54110 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX54110 Histone H3K79me2 (di-methyl Lys79) antibody.
Dilution : 1:200

Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in ChIP assay (ChIP assay). GTX54110

GTX54110 ChIP assay Image

ChIP analysis of 293 cell lysate using GTX54110 Histone H3K79me2 (di-methyl Lys79) antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54110

GTX54110 ICC/IF Image

ICC/IF analysis of 293T cells using GTX54110 Histone H3K79me2 (di-methyl Lys79) antibody.
Blue : DAPI

Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in Dot blot (Dot). GTX54110

GTX54110 Dot Image

Dot blot analysis of all sorts of methylation peptides using GTX54110 Histone H3K79me2 (di-methyl Lys79) antibody.

Anti-Histone H3K79me2 (di-methyl Lys79) antibody used in IHC (Paraffin sections) (IHC-P). GTX54110

GTX54110 IHC-P Image

IHC-P analysis of rat testis tissue using GTX54110 Histone H3K79me2 (di-methyl Lys79) antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399