Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade

ChIP analysis of sheared chromatin from 10⁶ HeLaS3 cells using GTX60336 Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the GAPDH promoter and for exon 2 of the inactive myoglobin gene. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K79me3 shows a preference for active promoters.

ELISA analysis of peptide containing the histone modification of interest using GTX60336 Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade.

ChIP analysis of sheared chromatin from 10⁶ HeLaS3 cells using GTX60336 Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade. The IP'd DNA was analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B), in a 2 Mb region from chromosome 11 (figure 2C), and in a 100 kb region surrounding the GAPDH positive control gene (figure 2D).
Antibody amount : 1μg

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60336 Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade.
Dilution : 1:1,000

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3K79 using GTX60336 Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade.
Dilution : 1:100,000