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Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade

Anti-Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade used in Dot blot (Dot). GTX60336
Anti-Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade used in Western Blot (WB). GTX60336
Anti-Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60336
Anti-Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade used in ELISA (ELISA). GTX60336
Anti-Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60336

Cat. No. GTX60336

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, Dot, ELISA, ChIP assay

Reactivity

 Human, Mouse
Package
50 μg ($429)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
Dot 1:100,000
ELISA 1:500
ChIP assay 1-5 μg
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Histone H3 containing the trimethylated lysine 79 (H3K79me3), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade used in Dot blot (Dot). GTX60336

GTX60336 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3K79 using GTX60336 Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade.
Dilution : 1:100,000

Anti-Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade used in Western Blot (WB). GTX60336

GTX60336 WB Image

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60336 Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade.
Dilution : 1:1,000

Anti-Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60336

GTX60336 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLaS3 cells using GTX60336 Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade. The IP'd DNA was analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the X-chromosome (figure 2A and B), in a 2 Mb region from chromosome 11 (figure 2C), and in a 100 kb region surrounding the GAPDH positive control gene (figure 2D).
Antibody amount : 1μg

Anti-Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade used in ELISA (ELISA). GTX60336

GTX60336 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60336 Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade.

Anti-Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60336

GTX60336 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLaS3 cells using GTX60336 Histone H3K79me3 (Tri-methyl Lys79) antibody - ChIP grade. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the GAPDH promoter and for exon 2 of the inactive myoglobin gene. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H3K79me3 shows a preference for active promoters.

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Package List Price ($)
$ 429