Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody

Cat. No. GTX02535

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all Histone H3 products

Datasheet
Reference

View product citations for antibody GTX02535 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:2000
ICC/IF	1:100-1:500
IHC-P	1:50-1:200

Not tested in other applications.

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Histone H3 around the acetylation site of Lys9/14/18/23/27.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX02535 IHC-P Image

IHC-P analysis of rat brain tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of mouse muscle tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of human gastric and adjacent normal cancer tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of human pancreatic cancer tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 WB Image

WB analysis of extracts from COLO205 cells (TSA 1M, 18 hr) using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.

GTX02535 IHC-P Image

IHC-P analysis of rat liver tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of human ovarian cancer tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of human colorectal cancer and adjacent normal tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of human colorectal cancer tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells (4h of LPS treatment) using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Red : Primary antibody
Green : Tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

GTX02535 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 WB Image

WB analysis of extracts from COLO205 cells (TSA 1M, 18 hr) using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Lane 1 : Ab prior treated with Ac-blocking peptide
Lane 2 : Ab prior treated with Non-Ac-blocking peptide
Lane 3 : untreated sample

GTX02535 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 WB Image

WB analysis of extracts from various samples using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Lane 1 : Heat-shock treated EC304 cells, blocked with antigen-specific peptides
Lane 2 : Heat-shock treated EC304 cells
Lane 3 : UV treated A2780 cells
Lane 4 : UV treated RAW264.7 cells

GTX02535 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of human esophageal and adjacent normal cancer tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of rat stomach tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of rat heart tissue using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX02535 IHC-P Image

IHC-P analysis of human pancreatic cancer and adjacent nomal tissues using GTX02535 Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

REFERENCE

Application Reference

Huang SH et al. Int J Mol Sci 2022; 23 (3) Alpha-Lipoic Acid Inhibits Spontaneous Diabetes and Autoimmune Recurrence in Non-Obese Diabetic Mice by Enhancing Differentiation of Regulatory T Cells and Showed Potential for Use in Cell Therapies for the Treatment of Type 1 Diabetes.

Application : WB

Submit a Reference

REVIEW

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Application Reference

Application : WB

Package	List Price ($)
100 μl	$ 399
Qty

Histone H3K9K14K23K27ac (Acetyl Lys9/Lys14/Lys23/Lys27) antibody

Cat. No. GTX02535

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Research Area

DATA IMAGES

GTX02535 IHC-P Image

GTX02535 IHC-P Image

GTX02535 IHC-P Image

GTX02535 IHC-P Image

GTX02535 IHC-P Image

GTX02535 WB Image

GTX02535 IHC-P Image

GTX02535 IHC-P Image

GTX02535 IHC-P Image

GTX02535 IHC-P Image

GTX02535 IHC-P Image

GTX02535 IHC-P Image

GTX02535 IHC-P Image

GTX02535 IHC-P Image

GTX02535 ICC/IF Image

GTX02535 IHC-P Image

GTX02535 WB Image

GTX02535 IHC-P Image

GTX02535 WB Image

GTX02535 IHC-P Image

GTX02535 IHC-P Image

GTX02535 IHC-P Image

GTX02535 IHC-P Image

GTX02535 IHC-P Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.