Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade

Cat. No. GTX60822

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, Dot, ELISA, ChIP assay, Protein Array
Reactivity	Human, Mouse, Zebrafish, Arabidopsis thaliana, Nematode, Aspergillus nidulans
Package	50 μg ($529)

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Datasheet

View product citations for antibody GTX60822 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1,000
ICC/IF	1:500
Dot	1:20,000
ELISA	1:100
ChIP assay	1-5 μg
Protein Array	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region of histone H3 containing the acetylated lysines 9 and 14 (H3K9/14ac), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60822 Protein Array Image

Protein Array analysis of an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade. This figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.
Dilution : 1:2,000

GTX60822 WB Image

WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade.
Dilution : 1:500

GTX60822 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3K9 using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade.
Dilution : 1:20,000

GTX60822 ChIP assay Image

ChIP analysis of sheared chromatin from 1x10⁵ K562 cells using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade. IgG (1 μg/IP) was used as a negative IP control. The IP'd DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and EIF4A2 genes, used as positive control targets, and the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative control targets (figure 2A). The IP'd DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence and a 1.5 Mb region of the X-chromosome (figure 2B and C) and in two regions surrounding the GAPDH and EIF4A2 positive control genes, respectively (figure 2D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow. These results clearly show an enrichment of the H3K9/14 acetylation at the promoters of active genes.
Antibody amount : 1μg

GTX60822 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade.

GTX60822 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

GTX60822 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:200

REFERENCE

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REVIEW

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Package

List Price ($)

50 μg

$ 529

Qty

Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade

Cat. No. GTX60822

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Research Area

DATA IMAGES

GTX60822 Protein Array Image

GTX60822 WB Image

GTX60822 Dot Image

GTX60822 ChIP assay Image

GTX60822 ELISA Image

GTX60822 ChIP assay Image

GTX60822 ICC/IF Image

REFERENCE

REVIEW

Product

Research

Support

Company

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