Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:200

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes GAPDH and EIF4A2, used as positive controls, and for the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

ELISA analysis of peptide containing the histone modification of interest using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade.

ChIP analysis of sheared chromatin from 1x10⁵ K562 cells using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade. IgG (1 μg/IP) was used as a negative IP control. The IP'd DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and EIF4A2 genes, used as positive control targets, and the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative control targets (figure 2A). The IP'd DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence and a 1.5 Mb region of the X-chromosome (figure 2B and C) and in two regions surrounding the GAPDH and EIF4A2 positive control genes, respectively (figure 2D and E). The position of the amplicon used for ChIP-qPCR is indicated by an arrow. These results clearly show an enrichment of the H3K9/14 acetylation at the promoters of active genes.
Antibody amount : 1μg

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3K9 using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade.
Dilution : 1:20,000

WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade.
Dilution : 1:500

Protein Array analysis of an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B using GTX60822 Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody - ChIP grade. This figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark.
Dilution : 1:2,000