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Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody

Anti-Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody used in Western Blot (WB). GTX122648
Anti-Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122648
Anti-Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody used in Western Blot (WB). GTX122648
Anti-Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody used in Dot blot (Dot). GTX122648

Cat No. GTX122648

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, Dot
Reactivity Human, Mouse
Package
100μl ($319),
25μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
Dot Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , HeLa (0.4 μM Trichostatin treatment for 18 hr)

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3K9K14ac (acetyl Lys9/Lys14). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Nucleus

Research Area

DATA IMAGES
Anti-Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody used in Western Blot (WB). GTX122648

GTX122648 WB Image

Histone H3K9K14ac (acetyl Lys9/Lys14) antibody detects Histone H3K9K14ac (acetyl Lys9/Lys14) protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H3K9K14ac (acetyl Lys9/Lys14) antibody (GTX122648) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122648

GTX122648 ICC/IF Image

Histone H3K9K14ac (acetyl Lys9/Lys14) antibody detects Histone H3K9K14ac (acetyl Lys9/Lys14) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K9K14ac (acetyl Lys9/Lys14) protein stained by Histone H3K9K14ac (acetyl Lys9/Lys14) antibody (GTX122648) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody used in Western Blot (WB). GTX122648

GTX122648 WB Image

Histone H3 (acetyl Lys 9, Lys14) antibody detects Histone H3 (acetyl Lys 9, Lys14) protein by western blot analysis.
A. 30 µg HeLa whole cell lysate/extract (untreated)
B. 30 µg HeLa whole cell lysate/extract (0.4 µM Trichostatin treatment for 18 hr)
15% SDS-PAGE
Histone H3 (acetyl Lys 9, Lys14) antibody (GTX122648) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3K9K14ac (Acetyl Lys9/Lys14) antibody used in Dot blot (Dot). GTX122648

GTX122648 Dot Image

Dotblot analysis of anti-Histone H3 (acetyl Lys 9, Lys14) antibody with peptide samples.
Peptide samples (0.1 µg) were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys 9, Lys14) antibody (GTX122648) at different dilution as indicated.
A: Peptide samples of Histone H3.1
B: Peptide samples of Histone H3 (acetyl Lys 9, Lys14)

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169