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Histone H3K9ac (Acetyl Lys9) antibody [AH3-120]

Cat. No. GTX12179

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AH3-120

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Frog
Package
50 μl ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2 μg/ml
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Positive Control

NIH3T3 treated with sodium butyrate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic, acetylated histone H3 peptide (amino acids 7-20, Ac-Lys9) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

RRID

AB_381384

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

REFERENCE

Application Reference

Sun Y et al. J Pain 2014; 15 (8) : 856-66 Opioids enhance CXCL1 expression and function after incision in mice.
Nair M et al. Dev Biol 2008; 320 (2) : 446-55 Nuclear regulator Pygo2 controls spermiogenesis and histone H3 acetylation.
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399