Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161] (1 ug/mL) as the detection antibody and GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.

The GTX60885 reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetyl-ated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.

ICC/IF analysis of HepG2 cells using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161].

Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody and biotinylated GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161] (1 ug/mL) as the detection antibody. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.

ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161].
Red : Primary antibody
Green : Actin

ChIP analysis of HeLa cells using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg

WB analysis of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161].
Dilution : 0.25μg/ml