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Histone H3K9ac (Acetyl Lys9) antibody

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178
Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178
Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178
Anti-Histone H3K9ac (Acetyl Lys9) antibody used in Western Blot (WB). GTX12178
Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178

Cat. No. GTX12178

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Frog
Package
50 μl ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:1,000
IHC-P Assay dependent

Note :

WB
20-40 μg using RIPA extracts (0.45 mg) of rat PC12 cells
Not tested in other applications.

Positive Control

NIH3T3 treated with sodium butyrate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2-2.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic acetylated [Ac-Lys9] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_381383

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178

GTX12178 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178

GTX12178 IHC-P Image

IHC-P analysis of rat lung tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178

GTX12178 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in Western Blot (WB). GTX12178

GTX12178 WB Image

WB analysis of NIH3T3 cells using GTX12178 Histone H3K9ac (acetyl Lys9) antibody.
Lane 1 : Anti Histone H3K9ac (acetyl Lys9) antibody
Lane 2 : Anti Histone H3K9ac (acetyl Lys9) + histone H3 (acetylated Lys9) peptide
Lane 3 : Anti Histone H3K9ac (acetyl Lys9) + histone H3 peptide(non-acetylated)

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178

GTX12178 IHC-P Image

IHC-P analysis of rat liver tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.

REFERENCE

Application Reference

Liang DY et al. Mol Pain 2014; 10 : 59 Epigenetic regulation of spinal cord gene expression controls opioid-induced hyperalgesia.
Application : IHC-P
Reactivity : Mouse
Ishiguro K et al. J Immunol 2011; 186 (5) : 295907 Cutting edge: tubulin α functions as an adaptor in NFAT-importin β interaction.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399