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Histone H3K9ac (Acetyl Lys9) antibody

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178
Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178
Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178
Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178
Anti-Histone H3K9ac (Acetyl Lys9) antibody used in Western Blot (WB). GTX12178

Cat No. GTX12178

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Frog
Package
50 μl ($279)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
ICC/IF 1:1,000
IHC-P Assay dependent

Note :

WB
20-40 μg using RIPA extracts (0.45 mg) of rat PC12 cells

Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

NIH3T3 treated with sodium butyrate

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2-2.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic acetylated [Ac-Lys9] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_381383

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H3 histone family member 3A , H3.3A , H3F3

Cellular Localization

Nucleus

Background

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Acetylation of lysine residues within these N-terminal domains by histone acetyl-transferases (HATs), including Gcn5p, P/CAF, p300/CBP, and TAFII250, is associated with transcriptional activation. This modification results in remodeling of the nucleosome structure into an open conformation more accessible to transcription complexes. Conversely, histone deacetylation by histone deacetylases (HDACs) is associated with transcription repression reversing the chromatin remodeling process. In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms.

Database

Research Area

DATA IMAGES

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178

GTX12178 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178

GTX12178 IHC-P Image

IHC-P analysis of rat liver tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178

GTX12178 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX12178

GTX12178 IHC-P Image

IHC-P analysis of rat lung tissue using GTX12178 Histone H3K9ac (acetyl Lys9) antibody at 0.1 μg/mL.

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in Western Blot (WB). GTX12178

GTX12178 WB Image

WB analysis of NIH3T3 cells using GTX12178 Histone H3K9ac (acetyl Lys9) antibody.
Lane 1 : Anti Histone H3K9ac (acetyl Lys9) antibody
Lane 2 : Anti Histone H3K9ac (acetyl Lys9) + histone H3 (acetylated Lys9) peptide
Lane 3 : Anti Histone H3K9ac (acetyl Lys9) + histone H3 peptide(non-acetylated)

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 279