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Histone H3K9ac (Acetyl Lys9) antibody

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX88007
Anti-Histone H3K9ac (Acetyl Lys9) antibody used in Western Blot (WB). GTX88007
Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX88007
Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Frozen sections) (IHC-Fr). GTX88007

Cat. No. GTX88007

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, EM

Reactivity

 Human, Mouse
Package
100 μg ($409)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
IHC-Fr Assay dependent
EM Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H3K9ac (1-50).

Purification

Purified by antigen-affinity chromatography. Non-acetylated peptide was removed by chromatography using corresponding non-acetylated peptide.
From serum

Conjugation

Unconjugated

RRID

AB_10731164

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX88007

GTX88007 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX88007 Histone H3K9ac (Acetyl Lys9) antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in Western Blot (WB). GTX88007

GTX88007 WB Image

WB analysis of Raw264.7 cells treated with TSA 400nM (24h) lysate using GTX88007 Histone H3K9ac (Acetyl Lys9) antibody. The lane on the right is blocked with the synthesized peptide.

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX88007

GTX88007 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX88007 Histone H3K9ac (Acetyl Lys9) antibody. The picture on the right is blocked with the synthesized peptide.

Anti-Histone H3K9ac (Acetyl Lys9) antibody used in IHC (Frozen sections) (IHC-Fr). GTX88007

GTX88007 IHC-Fr Image

The data was published in the 2022 in Int J Mol Sci. PMID: 35162978

REFERENCE

Application Reference

C Casali et al. Eur J Cell Biol 2023; 103 (1) : 151373 Impact of heat and cold shock on epigenetics and chromatin structure
Application : EM
Bankole O et al. Int J Mol Sci 2022; 23 (3) Beneficial and Sexually Dimorphic Response to Combined HDAC Inhibitor Valproate and AMPK/SIRT1 Pathway Activator Resveratrol in the Treatment of ALS Mice.
Application : IHC-P
Reactivity : Mouse
Yen YT et al. Nat Commun 2021; 12 (1) : 7297 Protein phosphatase 2A inactivation induces microsatellite instability, neoantigen production and immune response.
Application : WB
Reactivity : Mouse
Schiaffino L et al. Sci Rep 2018; 8 (1) : 12875 Acetylation state of RelA modulated by epigenetic drugs prolongs survival and induces a neuroprotective effect on ALS murine model.
Application : IHC-Fr
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409