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Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody

Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12181
Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in Western Blot (WB). GTX12181
Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX12181
Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX12181
Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX12181

Cat No. GTX12181

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Frog
Package
50 μl ($279)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
ICC/IF 1:1,000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

NIH3T3 treated with sodium butyrate

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic acetylated and phosphorylated [Ac-Lys9, pSer10] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H3 histone family member 3A , H3.3A , H3F3

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12181

GTX12181 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX12181 Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody at 1:500.Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton X-100.

Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in Western Blot (WB). GTX12181

GTX12181 WB Image

WB analysis of serum and sodium butyrate-treated NIH3T3 cell lysate using GTX12181 Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody.
Lane 1: Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody.
Lane 2: Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody + Histone H3(Ac-Lys9, pSer10) peptide
Lane 3: Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody + Histone H3 (non-Ac, non-phos) peptide
Lane 4: Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody+ Histone H3(Ac-Lys9) peptide
Lane 5: Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody:+ Histone H3(pSer10) peptide.

Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX12181

GTX12181 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX12181 Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody at 0.4 μg/mL.

Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX12181

GTX12181 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX12181 Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody at 0.2 μg/mL.

Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX12181

GTX12181 IHC-P Image

IHC-P analysis of rat duodenum tissue using GTX12181 Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody at 0.2 μg/mL.

REFERENCE

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REVIEW

There are currently no reviews for Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody (GTX12181). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 279