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Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody

Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12181
Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX12181
Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX12181
Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX12181
Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in Western Blot (WB). GTX12181

Cat. No. GTX12181

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Frog
Package
50 μl ($399)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:1,000
IHC-P Assay dependent
Not tested in other applications.

Positive Control

NIH3T3 treated with sodium butyrate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic acetylated and phosphorylated [Ac-Lys9, pSer10] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX12181

GTX12181 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX12181 Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody at 1:500.Cells were fixed and permeabilized with 4% PFA followed by 0.5% Triton X-100.

Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX12181

GTX12181 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX12181 Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody at 0.2 μg/mL.

Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX12181

GTX12181 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX12181 Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody at 0.4 μg/mL.

Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in IHC (Paraffin sections) (IHC-P). GTX12181

GTX12181 IHC-P Image

IHC-P analysis of rat duodenum tissue using GTX12181 Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody at 0.2 μg/mL.

Anti-Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody used in Western Blot (WB). GTX12181

GTX12181 WB Image

WB analysis of serum and sodium butyrate-treated NIH3T3 cell lysate using GTX12181 Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody.
Lane 1: Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody.
Lane 2: Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody + Histone H3(Ac-Lys9, pSer10) peptide
Lane 3: Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody + Histone H3 (non-Ac, non-phos) peptide
Lane 4: Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody+ Histone H3(Ac-Lys9) peptide
Lane 5: Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody:+ Histone H3(pSer10) peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399