Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H4 and H3 or unmodified histone H3 sequences using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody.
Dilution : 1:20,000

ChIP analysis of sheared chromatin from 1.5x10⁶ U2OS cells using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody. A titration of the antibody consisting of 5 and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the ALDOA (fructose-bisphosphate aldolase A) promoter and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

ICC/IF analysis of HeLa cells using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody.

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody.
Dilution : 1:250

ELISA analysis of peptide containing the histone modifications of interest using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody.