Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody

Cat. No. GTX60328

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, Dot, ELISA, ChIP assay
Reactivity	Human
Package	50 μl ($399)

See all Histone H3 products

Datasheet

View product citations for antibody GTX60328 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:250
ICC/IF	1:500
Dot	1:20,000
ELISA	1:1,000 - 1:4,000
ChIP assay	15 μl

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Histone H3 containing the acetylated lysine 9 and the phosphorylated serine 10 (H3K9acS10p), using a KLH-conjugated synthetic peptide.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX60328 ELISA Image

ELISA analysis of peptide containing the histone modifications of interest using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody.

GTX60328 WB Image

WB analysis of histone extracts (15 μg) from HeLa cells using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody.
Dilution : 1:250

GTX60328 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody.

GTX60328 ChIP assay Image

ChIP analysis of sheared chromatin from 1.5x10⁶ U2OS cells using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody. A titration of the antibody consisting of 5 and 15 μl per ChIP experiment was analysed. IgG (5 μg/IP) was used as negative IP control. QPCR was performed with primers for the ALDOA (fructose-bisphosphate aldolase A) promoter and for the coding region of the myogenic differentiation gene (MYOD), a gene that is inactive at normal conditions. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

GTX60328 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other modifications of histone H4 and H3 or unmodified histone H3 sequences using GTX60328 Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody.
Dilution : 1:20,000

REFERENCE

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REVIEW

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Package

List Price ($)

50 μl

$ 399

Qty

Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody

Cat. No. GTX60328

Host

Clonality

Isotype

Application

Reactivity